Immunofluorescent analysis of pan cytokeratin (pink) in MCF7 cells. Cells were fixed in ice-cold methanol for 10 minutes, and blocked with 10% BSA in PBS for 20 minutes at room temperature. Cells were probed with an APC-conjugated pan cytokeratin monoclonal antibody (Product # MA1-10325), diluted 1:200 in 3% BSA in PBS, for 1 hour at room temperature. Nuclei (blue) were stained with DAPI, and images were taken on a fluorescent microscope. Data courtesy of the Innovators Program.
|Tested species reactivity||Mammal|
|Published species reactivity||Rat, Fish, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Keratin-enriched preparation from human epidermoid carcinoma cell line A431.|
|Storage buffer||PBS with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18.
Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
New frontiers in circulating tumor cell analysis: A reference guide for biomolecular profiling toward translational clinical use.
MA1-10325 was used in immunocytochemistry to review the current state-of-the-art in circulating tumor cell profiling
|Becker TM,Caixeiro NJ,Lim SH,Tognela A,Kienzle N,Scott KF,Spring KJ,de Souza P||International journal of cancer (134:2523)||2014|
Characterization of goldfish fin cells in culture: some evidence of an epithelial cell profile.
MA1-10325 was used in immunocytochemistry to characterize goldfish fin cells through morphological, immunochemical and molecular methods
|Mauger PE,Labbé C,Bobe J,Cauty C,Leguen I,Baffet G,Le Bail PY||Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology (152:205)||2009|
Localization of progenitor/stem cells in the human tympanic membrane.
MA1-10325 was used in immunohistochemistry to identify the progenitor/stem cells in tympanic membrane
|Knutsson J,von Unge M,Rask-Andersen H||Audiology and neuro-otology (16:263)||2011|
Mind bomb 1 in the lymphopoietic niches is essential for T and marginal zone B cell development.
MA1-10325 was used in immunohistochemistry to study the essential role of mind bomb 1 in the development of T cells and marginal zone B cells
|Song R,Kim YW,Koo BK,Jeong HW,Yoon MJ,Yoon KJ,Jun DJ,Im SK,Shin J,Kong MP,Kim KT,Yoon K,Kong YY||The Journal of experimental medicine (205:2525)||2008|
Inhibition of VEGFR2 prevents DMBA-induced mammary tumor formation.
MA1-10325 was used in immunohistochemistry to investigate the effect of VEGFR2 blockade on breast cancer development stimulated by DMBA
|Heffelfinger SC,Yan M,Gear RB,Schneider J,LaDow K,Warshawsky D||Laboratory investigation; a journal of technical methods and pathology (84:989)||2004|
TNP-470 inhibits 7,12-dimethylbenz[a]anthracene-induced mammary tumor formation when administered before the formation of carcinoma in situ but is not additive with tamoxifen.
MA1-10325 was used in immunohistochemistry to investigate the effect of TNP-470 on breast tumor development stimulated with DMBA
|Heffelfinger SC,Gear RB,Schneider J,LaDow K,Yan M,Lu F,Pyle AL,Warshawsky D||Laboratory investigation; a journal of technical methods and pathology (83:1001)||2003|
Keratins modulate c-Flip/extracellular signal-regulated kinase 1 and 2 antiapoptotic signaling in simple epithelial cells.
MA1-10325 was used in western blot to study the role of keratins in modulating cFlip and ERK1/2 apoptotic signaling in epithelial cells
|Gilbert S,Loranger A,Marceau N||Molecular and cellular biology (24:7072)||2004|