|Tested species reactivity||Human|
|Published species reactivity||Rat, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human epidermal keratins from P3 x 63 Ag8 cells|
|Contains||0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Immunomicroscopy (IM)||Assay Dependent|
|Western Blot (WB)||1:1000 - 1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-5761 detects most human acidic (type I) keratins K10, K14, K15, K16, K19.
MA1-5761 has been successfully used in Western blot, immunomicroscopy and immunohistochemistry (paraffin) procedures. For Western blotting, do not use milk for blocking nonspecific binding because it contains some keratins (bovine serum albumin is recommended).
The MA1-5761 immunogen is human epidermal keratins.
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratin 5, 6 and 8 are members of the type II neutral-to-basic subfamily. Monoclonal antibodies to cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. PCK-26 facilitates the typing of normal, metaplastic and neoplastic cells and it may aid in the discrimination of carcinomas and non-epithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micro-metastases in lymph nodes and other tissues and for determining the origin of poorly differentiated tumors (1,2).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Association of Merkel cell polyomavirus infection with EGFR mutation status in Chinese non-small cell lung cancer patients.
MA1-5761 was used in immunohistochemistry to study Chinese non-small cell lung cancer patients to deterine if there is an association between polyomavirus infection of Merkel cells and EGFR hotspot mutations
|Xu S,Jiang J,Yu X,Sheng D,Zhu T,Jin M||Lung cancer (Amsterdam, Netherlands) (83:341)||2014|
A clinicopathological study of the significance of the proportion of choroid morphology in chordoid meningioma.
MA1-5761 was used in immunohistochemistry to study chordoid menigioma and the significance of the proportion of chordoid morphology for the risk of recurrence
|Lin JW,Lu CH,Lin WC,Wu YT,Huang YJ,Shih FY,Ho JT,Chuang MJ||Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia (19:836)||2012|
The prognostic impact of O6-methylguanine DNA methyltransferase and epidermal growth factor receptor expressions on primary gliosarcoma: a clinicopathologic and immunohistochemical study of seven cases at a single institution.
MA1-5761 was used in immunohistochemistry to study the prognostic value of MGMT and EGFR expression in primary gliosarcoma
|Lin JW,Wu YT,Chang IW||Indian journal of pathology and microbiology (54:683)||2012|
Rhabdoid papillary meningioma: a clinicopathologic case series study.
MA1-5761 was used in immunohistochemistry to study the clinicopathology of a series of cases of rhabdoid papillary meningioma
|Wu YT,Ho JT,Lin YJ,Lin JW||Neuropathology : official journal of the Japanese Society of Neuropathology (31:599)||2011|
Clear cell meningioma with frequent chordoid features and aggressive behavior: a clinicopathologic study of ten cases at a single institution.
MA1-5761 was used in immunohistochemistry to study the clinicopathology of clear cell meningioma with frequent chordoid features and aggressive behavior
|Chen HK,Wu YT,Lin YJ,Lin JW||Journal of neuro-oncology (103:551)||2011|
Novel immunofluorescence protocol for multimarker assessment of putative disseminating breast cancer stem cells.
MA1-5761 was used in immunohistochemistry to investigate the efficiency of a new method for the assessment of putative disseminating breast cancer stem cells
|Balic M,Rapp N,Stanzer S,Lin H,Strutz J,Szkandera J,Daidone MG,Samonigg H,Cote RJ,Dandachi N||Applied immunohistochemistry and molecular morphology : AIMM (19:33)||2011|
Chordoid meningioma: a clinicopathologic study of 11 cases at a single institution.
MA1-5761 was used in immunohistochemistry to study the clinicopathology of 11 cases of chordoid meningioma
|Lin JW,Ho JT,Lin YJ,Wu YT||Journal of neuro-oncology (100:465)||2010|
Melanotic Xp11 translocation renal cancer: a case with PSF-TFE3 gene fusion and up-regulation of melanogenetic transcripts.
MA1-5761 was used in immunohistochemistry to report on a case of melanotic Xp11 translocation renal cancer
|Chang IW,Huang HY,Sung MT||The American journal of surgical pathology (33:1894)||2009|
Coexistence of different tissue tumourigenesis in an N-methyl-N-nitrosourea-induced mammary carcinoma model: a histopathological report in Sprague-Dawley rats.
MA1-5761 was used in immunohistochemistry to investigate tumourigenesis in non-mammary tissues in the MNU-induced mammary tumour model
|Esendagli G,Yilmaz G,Canpinar H,Gunel-Ozcan A,Guc MO,Guc D||Laboratory animals (43:60)||2009|
Detection of micrometastases and skip metastases with ex vivo sentinel node mapping in carcinoma of the colon and rectum.
MA1-5761 was used in immunohistochemistry to study micrometastases and skip metastases in carcinoma of the colon and rectum
|Yagci G,Unlu A,Kurt B,Can MF,Kaymakcioglu N,Cetiner S,Tufan T,Sen D||International journal of colorectal disease (22:167)||2007|
Mediastinal intraoperative radioisotope sentinel lymph node mapping in non-small-cell lung cancer.
MA1-5761 was used in immunohistochemistry to develop a method to map sentinel lymph nodes in non-small cell lung cancer patients
|Atinkaya C,Ozlem Küçük N,Koparal H,Aras G,Sak SD,Ozdemir N||Nuclear medicine communications (26:717)||2005|
Ectopic thyroid in the gallbladder: report of a case.
MA1-5761 was used in immunohistochemistry to describe an unusual case of ectopic thyroid tissue
|Ihtiyar E,Isiksoy S,Algin C,Sahin A,Erkasap S,Yasar B||Surgery today (33:777)||2003|
Assessment of the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines.
MA1-5761 was used in western blot to study the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines
|Chang CY,Chiou PP,Chen WJ,Li YH,Yiu JC,Cheng YH,Chen SD,Lin CT,Lai YS||Research in veterinary science (88:285)||2010|
Possible association between HPV16 E7 protein level and cytokeratin 19.
MA1-5761 was used in western blot to study the association between HPV16 E7 protein levels and cytokeratin 19
|Favia G,Kanduc D,Lo Muzio L,Lucchese A,Serpico R||International journal of cancer (111:795)||2004|
An immunohistochemical study of feline endometrial adenocarcinoma.
MA1-5761 was used in immunohistochemistry - paraffin section to characterize feline endometrial adenocarcinomas immunohistochemically
|Gil da Costa RM,Santos M,Amorim I,Lopes C,Pereira PD,Faustino AM||Journal of comparative pathology (140:254)||2009|