|Tested species reactivity||Human|
|Published species reactivity||Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human epidermal keratins from P3 x 63 Ag8 cells|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunomicroscopy (IM)||Assay Dependent|
|Western Blot (WB)||1:1000-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-5762 reacts with all basic (type II) human keratins in human samples, including K1, K2, K3, K4, K5, K6, K7 and K8.
MA1-5762 has been successfully used in Western blot, immunohistochemistry and electron microscopy procedures.
The MA1-5762 immunogen is human epidermal keratins.
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratin 5, 6 and 8 are members of the type II neutral-to-basic subfamily. Monoclonal antibodies to cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. PCK-26 facilitates the typing of normal, metaplastic and neoplastic cells and it may aid in the discrimination of carcinomas and non-epithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micro-metastases in lymph nodes and other tissues and for determining the origin of poorly differentiated tumors (1,2).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Novel immunofluorescence protocol for multimarker assessment of putative disseminating breast cancer stem cells.
MA1-5762 was used in immunohistochemistry to investigate the efficiency of a new method for the assessment of putative disseminating breast cancer stem cells
|Balic M,Rapp N,Stanzer S,Lin H,Strutz J,Szkandera J,Daidone MG,Samonigg H,Cote RJ,Dandachi N||Applied immunohistochemistry and molecular morphology : AIMM (19:33)||2011|
Coexistence of different tissue tumourigenesis in an N-methyl-N-nitrosourea-induced mammary carcinoma model: a histopathological report in Sprague-Dawley rats.
MA1-5762 was used in immunohistochemistry to investigate tumourigenesis in non-mammary tissues in the MNU-induced mammary tumour model
|Esendagli G,Yilmaz G,Canpinar H,Gunel-Ozcan A,Guc MO,Guc D||Laboratory animals (43:60)||2009|
BMP4 signaling regulates formation of Hertwig's epithelial root sheath during tooth root development.
MA1-5762 was used in immunohistochemistry to study the role of BMP4 signaling in regulating the formation of Hertwig's epithelial root sheath during tooth root development
|Hosoya A,Kim JY,Cho SW,Jung HS||Cell and tissue research (333:503)||2008|
Graft-versus-host-like disease complicating thymoma: lack of AIRE expression as a cause of non-hereditary autoimmunity?
MA1-5762 was used in immunohistochemistry to report on three cases of graft-versus-host-like enterocolonopathy
|Offerhaus GJ,Schipper ME,Lazenby AJ,Montgomery E,Morsink FH,Bende RJ,Musler AR,van Lier RA,van Noesel CJ||Immunology letters (114:31)||2007|
Mediastinal intraoperative radioisotope sentinel lymph node mapping in non-small-cell lung cancer.
MA1-5762 was used in immunohistochemistry to develop a method to map sentinel lymph nodes in non-small cell lung cancer patients
|Atinkaya C,Ozlem Küçük N,Koparal H,Aras G,Sak SD,Ozdemir N||Nuclear medicine communications (26:717)||2005|
Cytokeratin expression in lichen amyloidosus and macular amyloidosis.
MA1-5762 was used in immunohistochemistry to investigate the expression of cytokeratins in lichen and macular amyloidosis and their diagnostic value
|Apaydin R,Gürbüz Y,Bayramgürler D,Müezzinoglu B,Bilen N||Journal of the European Academy of Dermatology and Venereology : JEADV (18:305)||2004|
Assessment of the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines.
MA1-5762 was used in western blot to study the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines
|Chang CY,Chiou PP,Chen WJ,Li YH,Yiu JC,Cheng YH,Chen SD,Lin CT,Lai YS||Research in veterinary science (88:285)||2010|
An immunohistochemical study of feline endometrial adenocarcinoma.
MA1-5762 was used in immunohistochemistry - paraffin section to characterize feline endometrial adenocarcinomas immunohistochemically
|Gil da Costa RM,Santos M,Amorim I,Lopes C,Pereira PD,Faustino AM||Journal of comparative pathology (140:254)||2009|