|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Homogenized fibrin clot.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-20025 detects D-Dimer in human samples.
MA1-20025 has been successfully used in ELISA and Western blot procedures. To be analyzed in sandwich ELISA, plasma must be diluted with 10 mM Tris/HCl buffer, pH 7.5, 1 M NaCl, and 0.1% Tween 20. To avoid non-specific binding, the final NaCl concentration in plasma samples must be 0.5 M or higher.
The MA1-20025 immunogen is homogenized fibrin clot.
Fibrinogen is the main protein of blood coagulation system. It consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. The process of blood coagulation results in the activation of fibrinogen into fibrin by thrombin and fibrin polymerization. Fibrin clot is then digested by plasmin, and fibrin degradation products of different molecular weights are released into the bloodstream.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.