Western blot analysis of D4GDI in Jurkat cells using a D4 GDI monoclonal antibody (Product # MA1-41181) at 2 ug/ml and a D4 GDI monoclonal antibody (Product # MA1-41085) at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. a D4 GDI monoclonal antibody (Product # MA1-41181) detects both the full-length and cleaved forms of D4GDI while a D4 GDI monoclonal antibody (Product # MA1-41085) specifically detects only the cleaved form of the protein.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||Synthetic peptide corresponding to a portion of human D4-GDI.|
|Storage buffer||PBS with 0.02% gelatin|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: antigen standard for ARHGDIB (transient overexpression lysate), Daudi or Jurkat.
D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and 5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.