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Product Details
60082-1-IG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3B6D2
Immunogen
DACH1 Fusion Protein Ag4474 (176-521 aa encoded by BC021219)
Conjugate
Form
Liquid
Concentration
0.83 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human DACH1.
Immunogen sequence: QHGADSENG DMNSSVGLEL PFMMMPHPLI PVSLPPASVT MAMSQMNHLS TIANMAAAAQ VQSPPSRVET SVIKERVPDS PSPAPSLEEG RRPGSHPSSH RSSSVSSSPA RTESSSDRIP VHQNGLSMNQ MLMGLSPNVL PGPKEGDLAG HDMGHESKRM HIEKDETPLS TPTARDSLDK LSLTGHGQPL PPGFPSPFLF PDGLSSIETL LTNIQGLLKV AIDNARAQEK QVQLEKTELK MDFLRERELR ETLEKQLAME QKNRAIVQKR LKKEKKAKRK LQEALEFETK RREQAEQTLK QAASTDSLRV LNDSLTPEIE ADRSGGRTDA ERTIQDGRLY LKTTVMY (176-521 aa encoded by BC021219 )
Target Information
Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1. Binds to chromatin DNA via its DACHbox-N domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: dac homolog; Dach1; dachshund family transcription factor 1; Dachshund homolog 1; FLJ10138; OTTHUMP00000040839; RP11-512J14.1
Gene Aliases: Dac; DACH; DACH1
UniProt ID: (Human) Q9UI36, (Mouse) Q9QYB2
Entrez Gene ID: (Human) 1602, (Rat) 306096, (Mouse) 13134
transcription factor activity, RNA polymerase II core promoter sequence-specific binding involved in preinitiation complex assembly
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
protein binding
transcription factor activity, sequence-specific DNA binding
winged helix/forkhead transcription factor
negative regulation of transcription from RNA polymerase II promoter
suckling behavior
multicellular organism development
respiratory gaseous exchange
cell proliferation
negative regulation of transcription by competitive promoter binding
negative regulation of cell migration
regulation of nuclear cell cycle DNA replication
negative regulation of transcription, DNA-templated
development of primary female sexual characteristics
negative regulation of fibroblast proliferation
RNA polymerase II transcriptional preinitiation complex assembly
negative regulation of cell proliferation involved in contact inhibition
negative regulation of DNA biosynthetic process
regulation of transcription, DNA-templated
transcription, DNA-templated
transcription from RNA polymerase II promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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