|Tested species reactivity||Mouse|
|Host / Isotype||Armenian hamster / IgG|
|Immunogen||GPI-DAF transfected CHO cells.|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||1 ug/1 million cells|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-70050 detects DAF in mouse samples.
MA1-70050 has been successfully used in flow cytometry, ELISA and Western blot procedures.
The MA1-70050 immunogen is GPI-DAF transfected CHO cells.
Signals from TGF-beta superfamily receptors are transduced to the nucleus by Smad proteins, which transcriptionally activate target genes. In Caenorhabditis elegans (C.elegans), defects in a TGF-beta-related pathway cause a reversible developmental arrest and metabolic shift at the dauer larval stage. DAF-3 transgenes interfere with dauer formation, playing a role in the nucleus. DAF-3 is unique in that it is antagonized, rather than activated, by a TGF-beta pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.