|Tested species reactivity||Human|
|Host / Isotype||Mouse|
|Immunogen||A recombinant His-tagged protein fragment corresponding to the extracellular region of human DC-SIGN.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||5-10 ug/ml|
|Western Blot (WB)||1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control is human uterus tissue lysate.
Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes , where DCs hand over HIV to CD4+ T lymphocytes. DCs also amplify the amount of virus and extend the duration of viral infectivity. Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC-SIGN. ICAM-3 is the natural ligand for DC-SIGN. A DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC-SIGN2) was identified recently. DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR are known to exisit (8). The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.