|Immunohistochemistry (IHC)||5 ug/ml|
|Western Blot (WB)||1-2 ug/ml|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 14 amino acid peptide near the carboxy terminus of human DCLK2.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
A suggested positive control is rat brain tissue lysate.
PA5-20910 can be used with blocking peptide PEP-1024.
DCLK2 is one of three doublecortin-like kinases similar to the Ca2+/calmodulin-dependent protein kinase (CaMK) family. DCLK2 mRNA, like that of the homologous DCLK1 and DCLK3, is highly expressed in adult brain, but only DCLK1 and DCLK2 transcripts are present in human fetal brain and the developing mouse embryo, suggesting that DCLK1 and DCLK2 may play roles in cortical development. The DCLK proteins are homologous to Doublecortin (DCX), a protein that is mutated in X-linked human lissencephaly. In mouse models where the DCX gene has been disrupted, DCLK1 expression increases slightly and appears to compensate for the loss of DCX, as mice mutant for both DCX and DCLK1 show a severe phenotype including perinatal lethality, disorganized neocortical layering, and profound hippocampal cytoarchitectural disorganization. Unlike DCLK1, DCLK2 expression does not change in DCX-null mice.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CaMK-like CREB regulatory kinase 2; CL2; CL2, CLICK-II, CLICK2, CLIK2, DCAMKL2, DCDC3, DCDC3B, DCK2; doublecortin and CaM kinase-like 2; Doublecortin domain-containing protein 3B; doublecortin kinase 2; Doublecortin-like and CAM kinase-like 2; Doublecortin-like kinase 2; Serine/threonine-protein kinase DCLK2
Gene Aliases: 6330415M09Rik; AU044875; CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2; DCDC3; DCDC3B; DCK2; DCLK2; RGD1308384
Molecular Function: non-receptor serine/threonine protein kinase
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