OriGene

DCLRE1B Monoclonal Antibody (OTI2B7), TrueMAB™

Product Image Gallery

DCLRE1B Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCLRE1B (RC205744, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-DCLR. Positive lysates LY411532 (100 µg) and LC411532 (20 µg) can be purchased separately from OriGene.

DCLRE1B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DCLRE1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502352)

DCLRE1B Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of Hela cells, using anti-DCLRE1B antibody (TA502352), (Red), compared to a nonspecific negative control antibody, (Blue).

DCLRE1B Antibody in Immunocytochemistry (ICC/IF) — Anti-DCLRE1B mouse monoclonal antibody (TA502352) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DCLRE1B(RC205744). (1:100)

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:500

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Flow Cytometry (Flow)

1:100

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI2B7

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-280 of human DCLRE1B produced in E.coli.

Conjugate

Unconjugated

Form

lyophilized

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product # 7KMWCO

Target Information

DNA interstrand cross-links prevent strand separation, thereby physically blocking transcription, replication, and segregation of DNA. DCLRE1B is one of several evolutionarily conserved genes involved in repair of interstrand cross-links.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: 5' exonuclease Apollo; DNA cross-link repair 1B (PSO2 homolog, S. cerevisiae); DNA cross-link repair 1B protein; PSO2 homolog; SNM1 homolog B; SNMIB

Gene Aliases: APOLLO; DCLRE1B; SNM1B; SNMIB

UniProt ID: (Human) Q9H816

Entrez Gene ID: (Human) 64858

Function(s)

cell cycle checkpoint telomere maintenance double-strand break repair via nonhomologous end joining telomeric loop formation protection from non-homologous end joining at telomere telomeric 3' overhang formation interstrand cross-link repair nucleic acid phosphodiester bond hydrolysis

Process(es)

damaged DNA binding protein binding 5'-3' exonuclease activity 5'-3' exodeoxyribonuclease activity