OriGene

DCLRE1B Monoclonal Antibody (OTI2B7), TrueMAB™

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DCLRE1B Antibody in Immunocytochemistry (ICC/IF) — Anti-DCLRE1B mouse monoclonal antibody (TA502352) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DCLRE1B(RC205744). (1:100)

DCLRE1B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human endometrium tissue within the normal limits using anti-DCLRE1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502352)

DCLRE1B Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCLRE1B (RC205744, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-DCLR. Positive lysates LY411532 (100 µg) and LC411532 (20 µg) can be purchased separately from OriGene.

DCLRE1B Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC205744 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DCLRE1B antibody (TA502352), and then analyzed by flow cytometry.

Product Details

CF502352

Applications	Tested Dilution	Publications
Western Blot (WB)	1:200-1:500	-
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-
Immunocytochemistry (ICC/IF)	1:100	-
Flow Cytometry (Flow)	1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI2B7
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-280 of human DCLRE1B produced in E.coli. View antigen
Conjugate	Unconjugated
Form	lyophilized
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.3, with 8% trehalose
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

DNA interstrand cross-links prevent strand separation, thereby physically blocking transcription, replication, and segregation of DNA. DCLRE1B is one of several evolutionarily conserved genes involved in repair of interstrand cross-links.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 5' exonuclease Apollo; DNA cross-link repair 1B (PSO2 homolog, S. cerevisiae); DNA cross-link repair 1B protein; PSO2 homolog; SNM1 homolog B; SNMIB

Gene Aliases: APOLLO; DCLRE1B; SNM1B; SNMIB

UniProt ID: (Human) Q9H816

Entrez Gene ID: (Human) 64858

Molecular Function: DNA metabolism protein