Invitrogen

DCLRE1B Monoclonal Antibody (OTI2B7)

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DCLRE1B Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of DCLRE1B in COS7 cells. Cells were transfected with a plasmid overexpressing DCLRE1B and probed with a DCLRE1B monoclonal antibody (Product # MA5-25400).

DCLRE1B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a DCLRE1B monoclonal antibody (Product # MA5-25400).

DCLRE1B Antibody in Western Blot (WB) — Western blot analysis of DCLRE1B in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with DCLRE1B (Product # MA5-25400) monoclonal antibody.

Product Details

MA5-25400

Applications	Tested Dilution	Publications
Western Blot (WB)	1:200-1:500	-
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-
Immunocytochemistry (ICC/IF)	Assay-dependent	-
Flow Cytometry (Flow)	1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI2B7
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-280 of DCLRE1B produced in E.coli View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.62 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2724194

Target Information

DNA interstrand cross-links prevent strand separation, thereby physically blocking transcription, replication, and segregation of DNA. DCLRE1B is one of several evolutionarily conserved genes involved in repair of interstrand cross-links.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 5' exonuclease Apollo; DNA cross-link repair 1B (PSO2 homolog, S. cerevisiae); DNA cross-link repair 1B protein; PSO2 homolog; SNM1 homolog B; SNMIB

Gene Aliases: APOLLO; DCLRE1B; SNM1B; SNMIB

UniProt ID: (Human) Q9H816

Entrez Gene ID: (Human) 64858

Molecular Function: DNA metabolism protein