Invitrogen

DCLRE1B Monoclonal Antibody (OTI2B7)

Product Image Gallery

DCLRE1B Antibody in Immunocytochemistry (ICC) — Immunofluorescent analysis of DCLRE1B in COS7 cells. Cells were transfected with a plasmid overexpressing DCLRE1B and probed with a DCLRE1B monoclonal antibody (Product # MA5-25400).

DCLRE1B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a DCLRE1B monoclonal antibody (Product # MA5-25400).

DCLRE1B Antibody in Western Blot (WB) — Western blot analysis of DCLRE1B in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with DCLRE1B (Product # MA5-25400) monoclonal antibody.

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1:100

Immunocytochemistry (ICC)

assay dependent

Immunofluorescence (IF)

assay dependent

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Western Blot (WB)

1:200-1:500

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI2B7

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-280 of DCLRE1B produced in E.coli

Conjugate

Unconjugated

Form

Liquid

Concentration

0.65 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2724194

Target Information

DNA interstrand cross-links prevent strand separation, thereby physically blocking transcription, replication, and segregation of DNA. DCLRE1B is one of several evolutionarily conserved genes involved in repair of interstrand cross-links.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 5' exonuclease Apollo; APOLLO; DNA cross-link repair 1B (PSO2 homolog; DNA cross-link repair 1B (PSO2 homolog, S. cerevisiae); DNA cross-link repair 1B protein; PSO2 homolog; S. cerevisiae); SNM1 homolog B; SNM1B; SNMIB

Gene Aliases: APOLLO; DCLRE1B; SNM1B; SNMIB

UniProt ID: (Human) Q9H816

Entrez Gene ID: (Human) 64858

Function(s)

cell cycle checkpoint telomere maintenance double-strand break repair via nonhomologous end joining telomeric loop formation protection from non-homologous end joining at telomere telomeric 3' overhang formation interstrand cross-link repair nucleic acid phosphodiester bond hydrolysis

Process(es)

damaged DNA binding protein binding 5'-3' exonuclease activity 5'-3' exodeoxyribonuclease activity