Western blot analysis was performed on whole cell extracts of MCF7 (Lane 1), T-47D (Lane 2), HeLa (Lane 3), HCT 116 (Lane 4), NIH/3T3 (Lane 5), Hep G2 (Lane 6). The blots were probed with Anti- DDB1 Mouse Monoclonal (Product # 37-6200, 2 ug/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 ug/ml, 1:2500 dilution). A ~ 130 kDa band corresponding to DDB1 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with Pierce™ Power Blotter System (Product # 22834). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant human DDB1 protein|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||2 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes the large subunit of DNA damage-binding protein which is a heterodimer composed of a large and a small subunit. This protein functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
HIV-1 and HIV-2 exhibit divergent interactions with HLTF and UNG2 DNA repair proteins.
37-6200 was used in western blot to analyze the divergent interactions with UNG2 and HLTF DNA repair proteins by HIV-1 and HIV-2
|Hrecka K,Hao C,Shun MC,Kaur S,Swanson SK,Florens L,Washburn MP,Skowronski J||Proceedings of the National Academy of Sciences of the United States of America (113:E3921)||2016|
CRL4A(CRBN) E3 ubiquitin ligase restricts BK channel activity and prevents epileptogenesis.
37-6200 was used in western blot to investigate how ubiquitination of BK channels contributes to epilepsy.
|Liu J,Ye J,Zou X,Xu Z,Feng Y,Zou X,Chen Z,Li Y,Cang Y||Nature communications (5:null)||2014|
|Not Applicable||Not Cited||
Lentiviral Vpx accessory factor targets VprBP/DCAF1 substrate adaptor for cullin 4 E3 ubiquitin ligase to enable macrophage infection.
37-6200 was used in western blot to suggest that lentiviral Vpx usurps VprBP-associated Cullin 4 ubiquitin ligase to allow for efficient reverse transcription
|Srivastava S,Swanson SK,Manel N,Florens L,Washburn MP,Skowronski J||PLoS pathogens (4:null)||2008|
|Human||Not Cited||Ubiquitin hydrolase UCH-L1 destabilizes mTOR complex 1 by antagonizing DDB1-CUL4-mediated ubiquitination of raptor.||Hussain S,Feldman AL,Das C,Ziesmer SC,Ansell SM,Galardy PJ||Molecular and cellular biology (33:1188)||2013|