Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF7 (Lane 1), T-47D (Lane 2), HeLa (Lane 3), HCT 116 (Lane 4), Hep G2 (Lane 5), NIH/3T3 (Lane 6), tissue extracts (30 µg lysate) of Rat Testis (Lane 7) and Mouse Testis (Lane 8). The blots were probed with Anti-DDB1 Mouse Monoclonal Antibody (Product # 39-9901, 1-3 µg/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conj µgate (Product # A28177, 0.4 µg/ml, 1:2500 dilution). A 126kDa band corresponding to DDB1 was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with overnight wet transfer system. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human, Mouse, Rabbit, Rat|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant protein encompassing the C-terminal region of the human DDB1 protein|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes the large subunit of DNA damage-binding protein which is a heterodimer composed of a large and a small subunit. This protein functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Nucleotide excision repair deficiency in melanoma in response to UVA.
39-9901 was used in western blot to study UVA response and nucleotide excision repair deficiency in melanoma
|Murray HC,Maltby VE,Smith DW,Bowden NA||Experimental hematology and oncology (5:null)||2016|
Efficient parvovirus replication requires CRL4Cdt2-targeted depletion of p21 to prevent its inhibitory interaction with PCNA.
39-9901 was used in immunocytochemistry and western blot to study the p21 degradation during parvovirus replication.
|Adeyemi RO,Fuller MS,Pintel DJ||PLoS pathogens (10:null)||2014|