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Invitrogen
DDB1 Monoclonal Antibody (ZD001)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
DDB1 Antibody (39-9901) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF7 (Lane 1), T-47D (Lane 2), HeLa (Lane 3), HCT 116 (Lane 4), Hep G2 (Lane 5), NIH/3T3 (Lane 6), tissue extracts (30 µg lysate) of Rat Testis (Lane 7) and Mouse Testis (Lane 8). The blots were probed with Anti-DDB1 Mouse Monoclonal Antibody (Product # 39-9901, 1-3 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conj µgate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 126 kDa band corresponding to ... View More
Product Details
39-9901
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rabbit,
Rat
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
ZD001
Immunogen
Recombinant protein encompassing the C-terminal region of the human DDB1 protein
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533443
Target Information
The DNA damage-binding protein from HeLa cells is associated with polypeptides of relative mass 124,000 (DDB1) and 41,000 (DDB2) as determined by SDS-polyacrylamide gels. To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, Keeney et al. (1994) injected purified human DDB protein into XPE cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: damage specific DNA binding protein 1; damage-specific DNA binding protein 1, 127kDa; Damage-specific DNA-binding protein 1; damage-specific DNA-binding protein, DNA repair; damaged-DNA recognition protein 1; DDB p127 subunit; DDBa; DNA damage-binding protein 1; DNA damage-binding protein a; DNA repair protein; HBV X-associated protein 1; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; UV-DDB 1; XAP-1; xeroderma pigmento; Xeroderma pigmentosum group E-complementing protein; XPCe; XPE-BF; XPE-binding factor
Gene Aliases: 127kDa; AA408517; DDB1; DDBA; p127-Ddb1; UV-DDB1; XAP1; XPCE; XPE; XPE-BF
UniProt ID: (Human) Q16531, (Mouse) Q3U1J4
Entrez Gene ID: (Rabbit) 100354475, (Human) 1642, (Mouse) 13194, (Rat) 64470
nucleotide-excision repair, DNA damage recognition
nucleotide-excision repair, DNA duplex unwinding
DNA repair
transcription-coupled nucleotide-excision repair
nucleotide-excision repair
nucleotide-excision repair, preincision complex stabilization
nucleotide-excision repair, preincision complex assembly
nucleotide-excision repair, DNA incision, 3'-to lesion
nucleotide-excision repair, DNA incision, 5'-to lesion
viral process
Wnt signaling pathway
nucleotide-excision repair, DNA incision
histone H2A monoubiquitination
DNA damage response, detection of DNA damage
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of viral genome replication
positive regulation by virus of viral protein levels in host cell
interaction with symbiont
global genome nucleotide-excision repair
UV-damage excision repair
regulation of mitotic cell cycle phase transition
positive regulation of viral release from host cell
cellular response to DNA damage stimulus
protein ubiquitination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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