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FIGURE: 1 / 1
DDX51 Antibody (PA5-90569) in WB
Western blot analysis of DDX51 in extracts of various cell lines. Samples were incubated with DDX51 Polyclonal antibody (Product # PA5-90569) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-90569
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 367-666 of human DDX51 (NP_7782362)
Conjugate
Form
Liquid
Concentration
4.86 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806122
Product Specific Information
Immunogen sequence: FLIIDEADRM IDSMHQSWLP RVVAAAFQSE DPADPCALLQ RRQAQAVTAA STCCPQMPLQ KLLFSATLTQ NPEKLQQLGL HQPRLFSTGL AHRGLEDTDG DGDSGKYAFP VGLTHHYVPC SLSSKPLVVL HLVLEMGFSR VLCFTNSREN SHRLFLLVQA FGGVDVAEFS SRYGPGQRRM ILKQFEQGKI QLLISTDATA RGIDVQGVEL VVNYDAPQYL RTYVHRVGRT ARAGKTGQAF TLLLKVQERR FLRMLTEAGA PELQRHELSS KLLQPLVPRY EEALSQLEES VKEERKQRAA; Positive Samples: HT-29, Jurkat, Mouse brain, Mouse spleen, Rat testis; Cellular Location: Nucleus, nucleolus
Target Information
ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits. [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent RNA helicase DDX51; DEAD (Asp-Glu-Ala-Asp) box polypeptide 51; DEAD box protein 51
Gene Aliases: 2310061O04Rik; AA537002; C81583; DDX51; R75505
UniProt ID: (Human) Q8N8A6, (Mouse) Q6P9R1
Entrez Gene ID: (Human) 317781, (Rat) 304570, (Mouse) 69663
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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