|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||NIH3T3 cells expressing full length Beta-glucan receptor.|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Neat-1:25|
|Functional Assay (FN)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01EU/ug.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CLEC7A, also known as dectin-1, is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily and is predominantly expressed on myeloid cells. It is a small glycoprotein type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif (ITAM). CLEC7A functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Upon fungal exposure, CLEC7A activates Syk tyrosine kinase, triggering a massive oxidative burst through the formation of reactive oxygen species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.