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DEFA1 Antibody (500-P126G-100UG) in WB
Western Blot: To detect Human NP-1 by Western Blot analysis DEFA1 Polyclonal Antibody (Product # 500-P126G-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human NP-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P126G-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human NP-1
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929360
Product Specific Information
AA Sequence of recombinant protein: ACYCRIPACI AGERRYGTCI YQGRLWAFCC.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human NP-1. Anti-Human NP-1-specific antibody was purified by affinity chromatography employing an immobilized Human NP-1 matrix.
Sandwich ELISA: To detect Human NP-1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human NP-1 (500-P126GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human NP-1.
Western Blot: To detect Human NP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human NP-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Defensin, alpha 1; defensin, alpha 1, myeloid-related sequence; defensin, alpha 2; HNP-1; HP-1; myeloid-related sequence; Neutrophil defensin 1
Gene Aliases: DEF1; DEFA1; DEFA1B; DEFA2; HNP-1; HP-1; HP1; MRS
UniProt ID: (Human) P59665
Entrez Gene ID: (Human) 1667
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