|Tested species reactivity||Horse, Human, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (243 DSPFCLRKQSHDNRKS 258) of DEPOR/DEPDC6 protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||3-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
DEP domain-containing mTOR-interacting protein (DEPOR/DEPTOR/DEPDC6) is a negative regulator of the mTORC1 and mTORC2 signaling pathways that regulate cell growth, proliferation and survival. It has two DEP domains and a PDZ (DHR) domain. Loss of DEPTOR activates S6K1, Akt, and SGK1, promotes cell growth and survival, and activates mTORC1 and mTORC2 kinase activities. DEPTOR overexpression suppresses S6K1 but, by relieving feedback inhibition from mTORC1 to PI3K signaling, activates Akt. Consistent with many human cancers having activated mTORC1 and mTORC2 pathways, DEPTOR expression is low in most cancers. Surprisingly, DEPTOR is highly overexpressed in a subset of multiple myelomas harboring cyclin D1/D3 or c-MAF/MAFB translocations. In these cells, high DEPTOR expression is necessary to maintain PI3K and Akt activation and a reduction in DEPTOR levels leads to apoptosis. It has also been reported that DEPOR has some functional significance in Lipid Biosynthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.