|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from N-terminus of human DFF-45 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is colon carcinoma.
Apoptosis is induced through a number of cell death receptors and their ligands like TNF and TNF receptor. The signaling cascade is carried further by adaptor proteins containing death domains and by caspase family of proteins. DFF-45 and DFF-40 (ICAD and CAD) are two subunits of heterodimeric caspase activated DNAse. DFF-45/ICAD (Inhibitor of Caspase Activated DNAse) acts as a chaperon for appropriate folding of DFF-40 and it also acts as inhibitor of DFF-40. Caspase 3 upon activation cleaves DFF to release DFF-40/CAD, which oligomerizes into a large active complex and eventually causes the degradation of DNA in nuclei. Hence cleavage of DFF-45 is an important step in apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.