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DGCR8 Monoclonal Antibody (4D11A3)

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Antibody Testing Data (11)

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DGCR8 Antibody (60084-1-IG) in WB

Human brain tissue were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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DGCR8 Antibody in Immunoprecipitation (IP) — IP result of anti-DGCR8 (IP:60084-1-IG, 5ug; Detection:60084-1-IG 1:500) with K-562 cells lysate 3440ug.

Product Details

60084-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 8 publications 8 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

RNA Immunoprecipitation (RIP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

4D11A3

Immunogen

DGCR8 Fusion Protein Ag4871 (167-517 aa encoded by BC009323)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: VKAKVEVCK DESVDLEEFR SYLEKRFDFE QVTVKKFRTW AERRQFNREM KRKQAESERP ILPANQKLIT LSVQDAPTKK EFVINPNGKS EVCILHEYMQ RVLKVRPVYN FFECENPSEP FGASVTIDGV TYGSGTASSK KLAKNKAARA TLEILIPDFV KQTSEEKPKD SEELEYFNHI SIEDSRVYEL TSKAGLLSPY QILHECLKRN HGMGDTSIKF EVVPGKNQKS EYVMACGKHT VRGWCKNKRV GKQLASQKIL QLLHPHVKNW GSLLRMYGRE SSKMVKQETS DKSVIELQQY AKKNKPNLHI LSKLQEEMKR LAEEREETRK KPKMSIVASA QPGGEPLCTV DV (167-517 aa encoded by BC009323)

Target Information

This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Lee J,Kang H

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Nucleolin Regulates Pulmonary Artery Smooth Muscle Cell Proliferation under Hypoxia by Modulating miRNA Expression.

Cells

Lee J,Kang H

Mon Mar 06 00:00:00 EST 2023

Splice site m⁶A methylation prevents binding of DGCR8 to suppress KRT4 pre-mRNA splicing in oral squamous cell carcinoma.

PeerJ

Li X,Fang J,Tao X,Xia J,Cheng B,Wang Y

Fri Feb 24 00:00:00 EST 2023

Hypoxia regulates overall mRNA homeostasis by inducing Met¹-linked linear ubiquitination of AGO2 in cancer cells.

Nature communications

Zhang H,Zhao X,Guo Y,Chen R,He J,Li L,Qiang Z,Yang Q,Liu X,Huang C,Lu R,Fang J,Cao Y,Huang J,Wang Y,Huang J,Chen GQ,Cheng J,Yu J

Mon Sep 13 00:00:00 EDT 2021

p38 MAPK-mediated loss of nuclear RNase III enzyme Drosha underlies amyloid beta-induced neuronal stress in Alzheimer's disease.

Aging cell

Xu H,Liu X,Li W,Xi Y,Su P,Meng B,Shao X,Tang B,Yang Q,Mao Z

Fri Oct 01 00:00:00 EDT 2021

Global miRNA dosage control of embryonic germ layer specification.

Nature

Cui Y,Lyu X,Ding L,Ke L,Yang D,Pirouz M,Qi Y,Ong J,Gao G,Du P,Gregory RI

Sat May 01 00:00:00 EDT 2021

DGCR8/ZFAT-AS1 Promotes CDX2 Transcription in a PRC2 Complex-Dependent Manner to Facilitate the Malignant Biological Behavior of Glioma Cells.

Molecular therapy : the journal of the American Society of Gene Therapy

Zhang F,Ruan X,Ma J,Liu X,Zheng J,Liu Y,Liu L,Shen S,Shao L,Wang D,Yang C,Cai H,Li Z,Feng Z,Xue Y

Wed Feb 05 00:00:00 EST 2020

DGCR8/ZFAT-AS1 Promotes CDX2 Transcription in a PRC2 Complex-Dependent Manner to Facilitate the Malignant Biological Behavior of Glioma Cells.

Molecular therapy : the journal of the American Society of Gene Therapy

Zhang F,Ruan X,Ma J,Liu X,Zheng J,Liu Y,Liu L,Shen S,Shao L,Wang D,Yang C,Cai H,Li Z,Feng Z,Xue Y

Wed Feb 05 00:00:00 EST 2020

SUMOylation at K707 of DGCR8 controls direct function of primary microRNA.

Nucleic acids research

Zhu C,Chen C,Huang J,Zhang H,Zhao X,Deng R,Dou J,Jin H,Chen R,Xu M,Chen Q,Wang Y,Yu J

Fri Sep 18 00:00:00 EDT 2015

SUMOylation at K707 of DGCR8 controls direct function of primary microRNA.

Nucleic acids research

Zhu C,Chen C,Huang J,Zhang H,Zhao X,Deng R,Dou J,Jin H,Chen R,Xu M,Chen Q,Wang Y,Yu J

Fri Sep 18 00:00:00 EDT 2015

SUMOylation at K707 of DGCR8 controls direct function of primary microRNA.

Nucleic acids research

Zhu C,Chen C,Huang J,Zhang H,Zhao X,Deng R,Dou J,Jin H,Chen R,Xu M,Chen Q,Wang Y,Yu J

Fri Sep 18 00:00:00 EDT 2015

Identification of microprocessor-dependent cancer cells allows screening for growth-sustaining micro-RNAs.

Oncogene

Peric D,Chvalova K,Rousselet G

Thu Apr 19 00:00:00 EDT 2012

Identification of microprocessor-dependent cancer cells allows screening for growth-sustaining micro-RNAs.

Oncogene

Peric D,Chvalova K,Rousselet G

Thu Apr 19 00:00:00 EDT 2012

Primary microRNA precursor transcripts are localized at post-synaptic densities in adult mouse forebrain.

Journal of neurochemistry

Lugli G,Larson J,Demars MP,Smalheiser NR

Thu Nov 01 00:00:00 EDT 2012

Primary microRNA precursor transcripts are localized at post-synaptic densities in adult mouse forebrain.

Journal of neurochemistry

Lugli G,Larson J,Demars MP,Smalheiser NR

Thu Nov 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: DiGeorge syndrome critical region 8; DiGeorge syndrome critical region 8 homolog; DiGeorge syndrome critical region gene 8; Gy1; Microprocessor complex subunit DGCR8

Gene Aliases: C22orf12; D16H22S1742E; D16H22S788E; D16Wis2; DGCR8; DGCRK6; Gy1; LP4941; mir-1306; N41; pasha; Vo59c07

UniProt ID: (Human) Q6DCB2, (Mouse) Q9EQM6

Entrez Gene ID: (Human) 54487, (Pig) 100616094, (Mouse) 94223, (Rat) 287954

Molecular Function: RNA metabolism protein RNA processing factor nucleic acid metabolism protein scaffold/adaptor protein