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DHFR Antibody (MA5-49170) in IHC (P)
Immunohistochemistry analysis of DHFR in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with DHFR Monoclonal antibody (Product # MA5-49170) using a dilution of 1 μg/mL overnight at 4°C. Biotinylated goat anti-mouse IgG was use... View More
Product Details
MA5-49170
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
3C8
Immunogen
E.coli-derived human DHFR recombinant protein (Position: V2-D187).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074828
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Human DHFR shares 90% amino acid (aa)sequence identity with both mouse and rat DHFR.
Positive Control - WB: human K562 whole cell, human SGC-7901 whole cell, human Hela whole cell, human SW620 whole cell, rat kidney tissue. IHC: human rectal cancer tissue, rat gaster tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, and is required for the synthesis of purines, thymidylic acid and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dihydrofolate reductase; Dihydrofolate reductase 1 (active)
Gene Aliases: DHFR; Dhfr1; DHFRP1; DYR
UniProt ID: (Human) P00374, (Rat) Q920D2
Entrez Gene ID: (Human) 1719, (Rat) 24312
G1/S transition of mitotic cell cycle
regulation of transcription involved in G1/S transition of mitotic cell cycle
glycine biosynthetic process
tetrahydrobiopterin biosynthetic process
one-carbon metabolic process
nucleotide biosynthetic process
axon regeneration
response to methotrexate
dihydrofolate metabolic process
tetrahydrofolate metabolic process
tetrahydrofolate biosynthetic process
folic acid metabolic process
positive regulation of nitric-oxide synthase activity
oxidation-reduction process
regulation of removal of superoxide radicals
response to nicotine
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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