|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Armenian hamster / IgG|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.25 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Description: The HMD1-5 monoclonal antibody reacts with mouse Delta-like 1, one of five type I transmembrane proteins that serves as a Notch receptor ligand. Upon binding the Notch receptor (e.g. Notch 1-4), Delta-like 1 undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases an intracellular fragment whose biological function remains controversial. Delta-like 1 is expressed by thymic stromal cells, as well as on macrophages, dendritic cells, and stromal cells in the spleen. This protein is also expressed in nonhematopoietic tissues such as the lung and brain. Delta-like 1 has been linked to B cell development and differentiation, especially marginal zone and plasma cells, regulation of splenic dendritic cells, and leukemogenesis.
Applications Reported: This HMD1-5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HMD1-5 antibody has been tested by flow cytometric analysis of mouse splenocytes and transfected cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
Delta-like protein 1 (Dlk1, DLL1) is a transmembrane protein characterized by epidermal growth factor (EGF)-like repeats. DLL1, which is also known as preadipocyte factor 1 (pref-1) because of its ability to inhibit preadipocyte differentiation, regulates the differentiation of several other cell types. It is a ligand for Notch receptor. It blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Differential regulation of splenic CD8- dendritic cells and marginal zone B cells by Notch ligands.||Sekine C,Moriyama Y,Koyanagi A,Koyama N,Ogata H,Okumura K,Yagita H||International immunology (21:295)||2009|
|Not Applicable||Not Cited||Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice.||Moriyama Y,Sekine C,Koyanagi A,Koyama N,Ogata H,Chiba S,Hirose S,Okumura K,Yagita H||International immunology (20:763)||2008|
|Not Applicable||Not Cited||Three-dimensional architecture of the thymus is required to maintain delta-like expression necessary for inducing T cell development.||Mohtashami M,Zúñiga-Pflücker JC||Journal of immunology (Baltimore, Md. : 1950) (176:730)||2006|
|Not Applicable||Not Cited||Delta-like 1 is necessary for the generation of marginal zone B cells but not T cells in vivo.||Hozumi K,Negishi N,Suzuki D,Abe N,Sotomaru Y,Tamaoki N,Mailhos C,Ish-Horowicz D,Habu S,Owen MJ||Nature immunology (5:638)||2004|