Invitrogen

DLX2 Monoclonal Antibody (HL1942)

View all (19) DLX2 antibodies

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DLX2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of DLX2 in DIV9 rat E18 primary cortical neuron and glia cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DLX2 stained by DLX2 monoclonal antibody (Product # MA5-47229) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.

DLX2 Antibody in Western Blot (WB) — Western blot analysis of DLX2 in 293T whole cell and nuclear extracts (30 µg) separated by 12% SDS-PAGE. Membrane was blotted with DLX2 Monoclonal antibody (Product # MA5-47229) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody. WCE: whole cell extract; NE: nuclear extract.

DLX2 Antibody in Western Blot (WB) — Western blot analysis of DLX2 in 293T whole cell extracts (30 µg) separated by 12% SDS-PAGE. Membrane was blotted with DLX2 Monoclonal antibody (Product # MA5-47229) using a dilution of 1:5,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody.

Product Details

MA5-47229

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

HL1942

Immunogen

Recombinant fragment of human DLX2

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Store as concentrated solution.

Centrifuge briefly prior to opening vial.

Target Information

Many vertebrate homeo box-containing genes have been identified on the basis of their sequence similarity with Drosophila developmental genes. Members of the Dlx gene family contain a homeobox that is related to that of Distal-less (Dll), a gene expressed in the head and limbs of the developing fruit fly. The Distal-less (Dlx) family of genes comprises at least 6 different members, DLX1-DLX6. The DLX proteins are postulated to play a role in forebrain and craniofacial development. This gene is located in a tail-to-tail configuration with another member of the gene family on the long arm of chromosome 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: distal-less homeo box 2; DLX-5; homeobox Dlx5; Homeobox protein DLX-2

Gene Aliases: DLX2; Dlx5; TES-1; TES1

UniProt ID: (Human) Q07687

Entrez Gene ID: (Human) 1746, (Rat) 296499

Molecular Function: homeodomain transcription factor

negative regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter brain development proximal/distal pattern formation subpallium development hippocampus development olfactory bulb development regulation of transcription from RNA polymerase II promoter involved in forebrain neuron fate commitment cerebral cortex GABAergic interneuron fate commitment odontogenesis of dentin-containing tooth negative regulation of Notch signaling pathway positive regulation of transcription from RNA polymerase II promoter embryonic cranial skeleton morphogenesis negative regulation of oligodendrocyte differentiation branching morphogenesis of a nerve cartilage development regulation of transcription from RNA polymerase II promoter forebrain neuron differentiation cerebral cortex GABAergic interneuron differentiation cell differentiation embryonic skeletal system development

Process(es)

RNA polymerase II regulatory region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding single-stranded RNA binding protein binding DNA binding sequence-specific DNA binding