|Tested species reactivity||Bacteria|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant DNA Adenine Methylase protein.|
|Contains||0.08% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||5 - 10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
DNA adenine methylase (DAM) is an enzyme that methylates adenine residues in the 4-base pair DNA sequence, GATC. This methylation reaction has been shown to be crucial in a variety of cellular processes in bacteria. These include (i) the control of initiation of DNA replication (GATC occurs 11 times in the highly conserved bacterial origin of replication sequence); (ii) methyldirected mismatch repair; (iii) control of transposition of certain transposable DNA elements; and (iv) control of gene expression. With regard to gene expression, the recent report by Heithoff et al. (Science; 284:967–970; 1999) showed that the deletion of the DAM gene rendered Salmonella typhimurium totally avirulent.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.