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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified, full length, native bovine protein.|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-26060 detects DNA Ligase I from human samples.
MA1-26060 has been successfully used in Western blot procedures.
The MA1-26060 immunogen is purified, full length, native bovine protein.
There are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repair and recombination. DNA ligase I migrates as a 125kDa phosphoprotein and is implicated in joining Okazaki fragments during DNA synthesis. It also has a role in the base and nucleotide excision repair pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Replication-dependent DNA damage response triggered by roscovitine induces an uncoupling of DNA replication proteins.
MA1-26060 was used in western blot to study the effects of the Cdk inhibitor roscovitine on DNA replication proteins
|Savio M,Cerri M,Cazzalini O,Perucca P,Stivala LA,Pichierri P,Franchitto A,Meijer L,Prosperi E||Cell cycle (Georgetown, Tex.) (5:2153)||2006|
Autoimmune responses to proliferating cell nuclear antigen multiprotein complexes involved in cell proliferation are strongly associated with their structure and biologic function in patients with systemic lupus erythematosus.
MA1-26060 was used in ELISA to study the mechanism of autoimmune responses to PCNA and its associated proteins in systemic lupus erythematosus
|Kogure T,Takasaki Y,Takeuchi K,Yamada H,Nawata M,Ikeda K,Matsushita M,Matsudaira R,Kaneda K,Terasawa K,Hashimoto H||Arthritis and rheumatism (46:2946)||2002|
ligase I, DNA, ATP-dependent; MGC117397; MGC130025; polydeoxyribonucleotide synthase [ATP] 1