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Western blot analysis of DNA Ligase III using A) 30 µg 293T whole cell lysate (B) 30 µg A431 whole cell lysate (C) 30 µg HeLa whole cell lysate and D) 30 µg HepG2 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a DNA Ligase III monoclonal antibody (Product # MA1-23191) at a dilution of 1:500.
|Tested species reactivity||Chicken, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant full-length human DNA Ligase III-b.|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Blocking Assay (BLOCK)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-23191 detects DNA ligase III in Human samples.
MA1-23191 has been successfully used in Western Blot, blocking and ICC/IF procedures. The predicted molecular weight of MA1-23191 is 100 kDa.
MA1-23191 immunogen corresponds to Recombinant full-length human DNA Ligase III-b.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
There are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase III gene product migrates as a 100kDa (a-form) protein in somatic tissues, is ubiquitously expressed and complexes with XRCC-1 and a 97kDa (ß-form) protein that is found in testis. DNA Ligase III is involved in rejoining DNA single-strand breaks and in the base excision repair pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
DNA ligase (ATP) 3; DNA ligase 3; DNA ligase III; LIG2; ligase II, DNA, ATP-dependent; ligase III, DNA, ATP-dependent; polydeoxyribonucleotide synthase [ATP] 3
D11Wsu78e; LIG2; LIG3