|Tested species reactivity||Human|
|Host / Isotype||Chicken / IgY|
|Immunogen||Purified recombinant DNMT3L.1 corresponding to the chromosome 21 gene (Human).|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-9542 detects DNMT3L in human samples.
PA1-9542 has been successfully used in Western blot procedures. By Western blot, PA1-9542 detects a 47 kDa protein representing DNMT3L.
PA1-9542 immunogen is a purified recombinant DNMT3L.1 corresponding to the chromosome 21 gene (Human).
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternative splicing results in two transcript variants. An additional splice variant has been described but its biological validity has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.