Western blot analysis using PA3-16558 to detect overexpressed DNMT-38 in HCT116 cells. Lane 1: 40 ug of nuclear extract from untransfected T24 degrees Cells. Lane 2-3: 40 ug of nuclear extracts transiently transfected to overexpress DNMT3B2 and DNMT3B3, respectively.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||This antibody was raised against amino acids 4-101 of the DNMT3b protein.|
|Storage buffer||whole serum|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody does not neutralize the enzymatic activity of its corresponding methyltransferase.
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Suggested positive control: HCT-116 cell lysates.
Inactivation of tumor suppressor genes is a key component of the development of all common forms of human cancers. Aberrant methylation may contribute to many of the changes, both physiological and pathological, associated with aging, including tumor development. Hypermethylation of CpG island-containing promoters is associated with the inactivation of genes important to tumor angiogenesis and progression. DNA methytransferase (DNMT3b) is necessary for the maintenance of the methylation process.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.