|Western Blot (WB)||1-3 µg/ml|
|Western Blot (WB)||See 4 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human , Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||DNA polymerase beta protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-12086 targets DNA Polymerase beta in WB applications and shows reactivity with Human samples.
The MA5-12086 immunogen is dNA polymerase beta protein.
DNA polymerase beta comprises an amino-terminal 8-kDa domain and a carboxy-terminal 31-kDa domain. The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity while the C-terminal domain has a nucleotidyltransferase activity. Mammalian DNA polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells, but treatment of cells with the DNA-alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulfonate up-regulates beta-pol level. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DNA pol beta; DNA polymerase beta; DNA polymerase beta subunit; DNA-directed DNA polymerase beta; Pol beta; polymerase (DNA directed), beta; polymerase (DNA) beta; polymerase-beta
Gene Aliases: A430088C08Rik; POLB
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