|Western Blot (WB)||5 µg/ml|
|Tested Species reactivity||Human, Mustelid, Mouse, Non-human primate|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Mono S FPLC column purified HeLa cell polymerase epsilon.|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
This antibody does not detect drosophilia or the baculovirus host cell pol epsilon, and weakly, if at all, on s.cerivisae pol epsilon.
DNA polymerase e from HeLa cells differs from yeast pol e in that HeLa pol e contains two subunits of ~220 and 55 kDa, whereas yeast pol e has one large subunit and three smaller subunits. Previous studies of template specificity of HeLa pol e, which appears to be required for long patch DNA repair in permeabilized cells, indicate pol e prefers long stretches of single-stranded regions in poly(dA)-oligo(dT) as template. HeLa pol a and pol d prefer
activated DNA and alternating poly(dA-dT) as templates, respectively. Pol e retains its processivity in the absence of proliferating cell nuclear antigen (PCNA), unlike pol d. PCNA can also interact with Pol e on linear DNA templates, even in the absence of other auxiliary factors (replication factor C, replication factor A), and thereby stimulate its primer recognition and DNA synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AsTP; 17; arsenic transactivated protein; Arsenic-transactivated protein; CHRAC 17; CHRAC-17; Chromatin Accessibility Complex 17; Chromatin accessibility complex 17 kDa protein; DNA Polymerase Epsilon p17 Subunit; DNA Polymerase Epsilon Subunit 3; DNA polymerase epsilon subunit p17; DNA polymerase epsilon, subunit 3; DNA polymerase II subunit 3; histone fold protein CHRAC17; huCHRAC17; NF-YB-like protein; polymerase (DNA directed), epsilon 3 (p17 subunit); polymerase (DNA directed), epsilon 3, accessory subunit; polymerase (DNA) epsilon 3, accessory subunit; YB-like protein 1; YBL1
Gene Aliases: 1810034K18Rik; CHARAC17; CHRAC17; p17; POLE3; YBL1
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.