|Tested species reactivity||Human, Mustelid, Mouse, Non-human primate|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Mono S FPLC column purified HeLa cell polymerase epsilon.|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody does not detect drosophilia or the baculovirus host cell pol epsilon, and weakly, if at all, on s.cerivisae pol epsilon.
DNA polymerase e from HeLa cells differs from yeast pol e in that HeLa pol e contains two subunits of ~220 and 55 kDa, whereas yeast pol e has one large subunit and three smaller subunits. Previous studies of template specificity of HeLa pol e, which appears to be required for long patch DNA repair in permeabilized cells, indicate pol e prefers long stretches of single-stranded regions in poly(dA)-oligo(dT) as template. HeLa pol a and pol d prefer
activated DNA and alternating poly(dA-dT) as templates, respectively. Pol e retains its processivity in the absence of proliferating cell nuclear antigen (PCNA), unlike pol d. PCNA can also interact with Pol e on linear DNA templates, even in the absence of other auxiliary factors (replication factor C, replication factor A), and thereby stimulate its primer recognition and DNA synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.