|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified native human recombinant amino acids 714 - 1061 DNA polymerase gamma.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
PA1-21791 detects DNA Polymerase gamma in Human samples.
PA1-21791 has been successfully used in and Western Blot procedures. Positive control of HeLa cells suggested.
PA1-21791 immunogen is purified native human recombinant amino acids 714 - 1061 DNA polymerase gamma.
As the only DNA polymerase (pol) present in mitochondria, DNA polymerase gamma (POLG) is necessarily implicated in base excision repair processes to correct oxidative damage to the mitochondrial genome. Therefore, the ability of the catalytic subunit of human POLG has been tested to participate in uracil provoked base excision repair reconstituted in vitro with purified components. Subsequent to actions of uracil DNA glycosylase and apurinic/apyrimidinic endonuclease, human POLG is able to fill a single nucleotide gap in the presence of a 5' terminal deoxyribose phosphate (dRP) flap. The catalytic subunit of human pol gamma catalyzes release of the dRP residue from incised apurinic/apyrimidinic sites to produce a substrate for DNA ligase. Faulty replication of the human mitochondrial genome is thought to be the cause of many diseases. The low selectivity of the mitochondrial DNA polymerase is implicated as the cause of many side effects observed in the treatment of viral infections such as HIV.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
TP53 codon 72 polymorphism affects accumulation of mtDNA damage in human cells.
PA1-21791 was used in immunoprecipitation and western blot to investigate the effects of different p53 isoforms on mitochondrial DNA damage
|Altilia S,Santoro A,Malagoli D,Lanzarini C,Ballesteros Álvarez JA,Galazzo G,Porter DC,Crocco P,Rose G,Passarino G,Roninson IB,Franceschi C,Salvioli S||Aging (4:28)||2012|