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Immunofluorescent analysis of DNA ligase 3 showing staining in the nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a DNA ligase 3 polyclonal antibody (Product # PA5-21480) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 116 and 433 of DNA ligase 3 (Uniprot ID#P49916)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-21480 targets DNA ligase 3 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-21480 immunogen is a recombinant fragment corresponding to a region within amino acids 116 and 433 of DNA ligase 3 (Uniprot ID#P49916).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ATP-dependent; DNA; LIG2; ligase II; ligase II, DNA, ATP-dependent; ligase III, DNA, ATP-dependent; polydeoxyribonucleotide synthase [ATP] 3