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Product Details
PA5-28903
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:3,000 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:100-1:1,000 | - |
Immunocytochemistry (ICC/IF) |
1:100-1:1,000 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant fragment corresponding to a region within amino acids 558 and 823 of Human DNA ligase IV |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
0.1M tris glycine, pH 7, with 20% glycerol |
Contains |
0.01% thimerosal |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2546379 |
Product Specific Information
Recommended positive controls: U87-MG, mouse brain, rat brain.
Predicted reactivity: Mouse (82%), Rat (81%), Sheep (83%), Bovine (86%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA joinase; DNA ligase (ATP) 4; DNA ligase 4; DNA ligase IV; DNA repair enzyme; ligase IV, DNA, ATP-dependent; Polydeoxyribonucleotide synthase [ATP] 4; polynucleotide ligase; sealase
Gene Aliases: 5830471N16Rik; LIG4; LIG4S; tiny
UniProt ID: (Human) P49917, (Mouse) Q8BTF7
Entrez Gene ID: (Human) 3981, (Rat) 290907, (Mouse) 319583
Molecular Function:
DNA metabolism protein
single strand break repair
in utero embryonic development
pro-B cell differentiation
DNA ligation
lagging strand elongation
nucleotide-excision repair, DNA gap filling
double-strand break repair
double-strand break repair via nonhomologous end joining
cell cycle
central nervous system development
cell proliferation
response to X-ray
response to gamma radiation
T cell differentiation in thymus
V(D)J recombination
immunoglobulin V(D)J recombination
T cell receptor V(D)J recombination
somatic stem cell population maintenance
negative regulation of neuron apoptotic process
isotype switching
positive regulation of fibroblast proliferation
positive regulation of neurogenesis
DNA ligation involved in DNA recombination
DNA ligation involved in DNA repair
chromosome organization
cell division
neuron apoptotic process
cellular response to lithium ion
DNA biosynthetic process
establishment of integrated proviral latency
double-strand break repair via classical nonhomologous end joining
positive regulation of chromosome organization
DNA repair
nucleotide-excision repair
response to ionizing radiation
DNA replication
DNA recombination
cellular response to DNA damage stimulus
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