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Invitrogen
DNA ligase IV Recombinant Rabbit Monoclonal Antibody (JM64-32)
Product Details
MA5-32775
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JM64-32
Immunogen
Recombinant protein within Human DNA Ligase IV aa 484-705
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2810052
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DNA joinase; DNA ligase 4; DNA ligase IV; DNA repair enzyme; ligase IV, DNA, ATP-dependent; Polydeoxyribonucleotide synthase [ATP] 4; polynucleotide ligase; sealase
Gene Aliases: LIG4; LIG4S
UniProt ID: (Human) P49917
Entrez Gene ID: (Human) 3981
single strand break repair
in utero embryonic development
pro-B cell differentiation
DNA ligation
lagging strand elongation
nucleotide-excision repair, DNA gap filling
double-strand break repair
double-strand break repair via nonhomologous end joining
cell cycle
central nervous system development
cell proliferation
response to X-ray
response to gamma radiation
T cell differentiation in thymus
V(D)J recombination
immunoglobulin V(D)J recombination
T cell receptor V(D)J recombination
somatic stem cell population maintenance
negative regulation of neuron apoptotic process
isotype switching
positive regulation of fibroblast proliferation
positive regulation of neurogenesis
DNA ligation involved in DNA recombination
DNA ligation involved in DNA repair
chromosome organization
cell division
neuron apoptotic process
cellular response to lithium ion
DNA biosynthetic process
establishment of integrated proviral latency
double-strand break repair via classical nonhomologous end joining
positive regulation of chromosome organization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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