Invitrogen

DNA ligase IV Recombinant Rabbit Monoclonal Antibody (JM64-32)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Reference

View all (15) DNA Ligase IV antibodies

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Datasheet

Protocols

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DNA ligase IV Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of DNA ligase IV of paraffin-embedded Human colon cancer tissue using a DNA-ligase-IV Monoclonal antibody (Product #MA5-32775). Counter stained with hematoxylin.

DNA ligase IV Antibody in Western Blot (WB) — Western blot was performed using Anti-DNA Ligase IV Recombinant Rabbit Monoclonal Antibody (JM64032) (Product # MA5-32775) and a 103 kDa band corresponding to LIG4 was observed across all the cell lines and tissues tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of MCF7 (Lane 1), HeLa (Lane 2), HEK-293 (Lane 3) and tissue extracts of Mouse Liver (Lane 4) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

DNA ligase IV Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with LIG4 siRNA and decrease in signal intensity was observed in western blot application using Anti-DNA Ligase IV Recombinant Rabbit Monoclonal Antibody (JM64-32) (Product # MA5-32775). {KD}

Product Details

MA5-32775

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (IHC)	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:50	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	JM64-32
Immunogen	Recombinant protein within Human DNA Ligase IV aa 484-705 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2810052

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

BMI1 nuclear location is critical for RAD51-dependent response to replication stress and drives chemoresistance in breast cancer stem cells.

Cell death & disease

Azzoni V,Wicinski J,Macario M,Castagné M,Finetti P,Ambrosova K,Rouault CD,Sergé A,Farina A,Agavnian E,Coslet S,Josselin E,Guille A,Adelaide J,Zacharioudakis E,Castellano R,Bertucci F,Birnbaum D,Rodriguez R,Charafe-Jauffret E,Ginestier C

MA5-32775 was used in Immunocytochemistry to show that breast cancer stem cells present a limited level of replication stress compared with non-breast cancer stem cells in patient samples.

Wed Feb 02 00:00:00 EST 2022

Bioinformatics

Protein Aliases: DNA joinase; DNA ligase 4; DNA ligase IV; DNA repair enzyme; ligase IV, DNA, ATP-dependent; Polydeoxyribonucleotide synthase [ATP] 4; polynucleotide ligase; sealase

Gene Aliases: LIG4; LIG4S

UniProt ID: (Human) P49917

Entrez Gene ID: (Human) 3981

Molecular Function: DNA metabolism protein

Function(s)

single strand break repair in utero embryonic development pro-B cell differentiation DNA ligation lagging strand elongation nucleotide-excision repair, DNA gap filling double-strand break repair double-strand break repair via nonhomologous end joining cell cycle central nervous system development cell proliferation response to X-ray response to gamma radiation T cell differentiation in thymus V(D)J recombination immunoglobulin V(D)J recombination T cell receptor V(D)J recombination somatic stem cell population maintenance negative regulation of neuron apoptotic process isotype switching positive regulation of fibroblast proliferation positive regulation of neurogenesis DNA ligation involved in DNA recombination DNA ligation involved in DNA repair chromosome organization cell division neuron apoptotic process cellular response to lithium ion DNA biosynthetic process establishment of integrated proviral latency double-strand break repair via classical nonhomologous end joining positive regulation of chromosome organization

Process(es)

DNA binding DNA ligase activity DNA ligase (ATP) activity protein binding ATP binding protein C-terminus binding ligase activity metal ion binding

DNA ligase IV Recombinant Rabbit Monoclonal Antibody (JM64-32)

Product Details

MA5-32775

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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