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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 1805 and 1899 of Human DNA polymerase IIA|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28087 targets DNA polymerase IIA in WB applications and shows reactivity with Human samples.
The PA5-28087 immunogen is synthetic peptide corresponding to a region within amino acids 1805 and 1899 of Human DNA polymerase IIA.
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
DNA-directed RNA polymerase II largest subunit; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; hRPB220; hsRPB1; POLR2; POLRA; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; RNA polymerase II 220 kd subunit; RNA polymerase II subunit B1; RNA-directed; RNA-directed RNA polymerase II subunit RPB1; RPB1; RPBh1; RpIILS; RPO2; RPOL2
hRPB220; hsRPB1; POLR2; POLR2A; POLRA; RPB1; RPBh1; RpIILS; RPO2; RPOL2