|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 122 and 396 of Human DNA polymerase iota|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-29442 targets DNA polymerase iota in WB applications and shows reactivity with Human samples.
The PA5-29442 immunogen is recombinant fragment corresponding to a region within amino acids 122 and 396 of Human DNA polymerase iota.
Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DNA polymerase iota; Eta2; iota; polymerase (DNA directed) iota; polymerase (DNA) iota; polymerase (DNA-directed); polymerase (DNA-directed), iota; RAD30 homolog B; RAD30B; RAD3OB
Gene Aliases: POLI; RAD30B; RAD3OB
UniProt ID: (Human) Q9UNA4
Entrez Gene ID: (Human) 11201
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