Invitrogen

DNAJA2 Monoclonal Antibody (OTI2A2)

Product Image Gallery

DNAJA2 Antibody in Western Blot (WB) — Western blot analysis of DNAJA2 in HepG2, HeLa, HT29, A549, COS7, Jurkat, MDCK, PC12, MCF7 cells using 35 µg per lane. Samples were probed with DNAJA2 (Product # MA5-25236) monoclonal antibody.

DNAJA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human lung tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a DNAJA2 monoclonal antibody (Product # MA5-25236) at a dilution of 1:50.

DNAJA2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of DNAJA2 in COS7 cells. Cells were transfected with a plasmid overexpressing DNAJA2 and probed with a DNAJA2 monoclonal antibody (Product # MA5-25236).

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2000

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Flow Cytometry (Flow)

1:100

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Dog, Human, Non-human primate, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI2A2

Immunogen

Full length human recombinant protein of DNAJA2 produced in HEK293T cell

Conjugate

Unconjugated

Form

Liquid

Concentration

0.49 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2723074

Target Information

The protein encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cell cycle progression 3 protein; Cell cycle progression restoration gene 3 protein; Dj3; DnaJ (Hsp40) homolog, subfamily A, member 2; DnaJ homolog subfamily A member 2; Dnj3; HIRA interacting protein 4; HIRA-interacting protein 4; Renal carcinoma antigen NY-REN-14

Gene Aliases: CPR3; DJ3; DJA2; DNAJ; DNAJA2; DNJ3; HIRIP4; PRO3015; RDJ2

UniProt ID: (Human) O60884

Entrez Gene ID: (Human) 10294, (Dog) 478143, (Rat) 84026

Function(s)

response to heat carbon tetrachloride metabolic process protein refolding positive regulation of cell proliferation

Process(es)

glycoprotein binding protein binding ATP binding heat shock protein binding metal ion binding unfolded protein binding chaperone binding