Western blot analysis of Cysteine String Protein alpha was performed using whole cell lysates of SH-SY5Y (Lane 1) and NIH/3T3 (Lane 2). The blots were probed with Anti- Cysteine String Protein alpha Rabbit polyclonal Antibody (Product # PA1-776, 2 µg/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/ml, 1:2500 dilution). Bands of ~ 35 and 26 kDa, corresponding to mature and immature forms of Cysteine String Protein alpha respectively, were observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues E(180) T T Q L T A D S H P S Y H T D G F N(198) of rat CSP.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-776 detects Cysteine String Protein Alpha in mouse and rat brain samples.
PA1-776 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~34 kDa protein representing Cysteine String Protein Alpha in rat and mouse brain homogenates.
The PA1-776 immunogen is a synthetic peptide corresponding to residues E(180) T T Q L T A D S H P S Y H T D G F N(198) of rat CSP. This sequence is conserved across many species including mouse, human and bovine. This peptide (Cat. # PEP-287) is available for use in neutralization and control experiments.
Cysteine string protein alpha (CSPalpha) is a synaptic vesicle protein that contains a DNA-J domain characteristic of Hsp40 chaperones. Cysteine-string proteins are membrane-bound proteins associated primarily with nerve endings and synaptic vesicles in the brain. These proteins play an important role in presynaptic function and may be involved in calcium-dependent neurotransmitter release. CSP proteins may act as presynaptic chaperones that assist in maintaining synaptic function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.