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Invitrogen
DNMT3A Monoclonal Antibody (64B1446)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 11
DNMT3A Antibody (MA5-16171) in IHC (P)
Immunohistochemical analysis of DNMT3A in human hepatocellular carcinoma tissue section. Samples were incubated in DNMT3A monoclonal antibody (Product # MA5-16171) using a dilution of 1:100. The antibody generated a specific cytoplasmic staining in all the cancer cells while some of the cells depicted nuclear staining also.
Product Details
MA5-16171
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Gerbil
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
64B1446
Immunogen
Bacteria expressed recombinant mouse Dnmt3a.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2537690
Product Specific Information
In Western blot, this antibody detects a band at approximately 120 kDa (predicted molecular weight: 102 kDa).
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Suggested positive control: antigen standard for DNMT3A (transient overexpression lysate), transfected cell lysate (WB), HeLa cells (ICC).
Target Information
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AU015806; Cysteine methyltransferase DNMT3A; DNA (cytosine-5)-methyltransferase 3A; DNA (cytosine-5-)-methyltransferase 3 alpha; DNA cytosine methyltransferase 3A2; DNA methyltransferase 3A; DNA methyltransferase HsaIIIA; DNA methyltransferase MmuIIIA; DNA MTase HsaIIIA; DNA MTase MmuIIIA; Dnmt3a; W91664
Gene Aliases: DNMT3A; DNMT3A2; M.HsaIIIA; MmuIIIA; TBRS
UniProt ID: (Human) Q9Y6K1, (Mouse) O88508, (Rat) Q1LZ53
Entrez Gene ID: (Human) 1788, (Mouse) 13435, (Rat) 444984
DNA binding
chromatin binding
DNA (cytosine-5-)-methyltransferase activity
protein binding
DNA-methyltransferase activity
identical protein binding
unmethylated CpG binding
metal ion binding
DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates
methyltransferase activity
transferase activity
DNA methyltransferase
negative regulation of transcription from RNA polymerase II promoter
mitotic cell cycle
DNA methylation
methylation-dependent chromatin silencing
regulation of gene expression by genetic imprinting
spermatogenesis
aging
response to toxic substance
response to ionizing radiation
response to lead ion
DNA methylation on cytosine within a CG sequence
positive regulation of cell death
neuron differentiation
response to estradiol
response to vitamin A
response to antidepressant
response to cocaine
DNA methylation involved in embryo development
DNA methylation involved in gamete generation
hypermethylation of CpG island
negative regulation of gene expression, epigenetic
S-adenosylhomocysteine metabolic process
S-adenosylmethioninamine metabolic process
cellular response to amino acid stimulus
cellular response to ethanol
cellular response to hypoxia
C-5 methylation of cytosine
hepatocyte apoptotic process
transcription, DNA-templated
regulation of transcription, DNA-templated
maintenance of DNA methylation
chromatin modification
methylation
DNA methylation on cytosine
response to nutrient levels
response to drug
response to ethanol
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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