|Tested species reactivity||Human, Mouse|
|Published species reactivity||Fruit fly|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues D(86) L L P N G D L E K R S E P Q(100) C of human Dnmt3a.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
PA1-882 detects DNA methyltransferase 3a (Dnmt3a) from human and mouse tissues and cells as well as recombinant human Dnmt3a. This antibody does not detect full length recombinant human Dnmt3b or Dnmt1.
PA1-882 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~120 kDa protein representing Dnmt3a from P19 cell nuclear extracts.
The PA1-882 immunogen is a synthetic peptide corresponding to residues D(86) L L P N G D L E K R S E P Q(100) C of human Dnmt3a. This immunizing peptide (Cat. # PEP-116) is available for use in neutralization and control experiments.
DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, genetic imprinting, and X-chromosome inactivation. Hypermethylation of DNA has also been implicated in tumorigenesis through improper methylation of growth regulatory gene promoter regions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Fruit fly||Not Cited||
Preferential methylation of unmethylated DNA by Mammalian de novo DNA methyltransferase Dnmt3a.
PA1-882 was used in immunoprecipitation to investigate whether the DNA methyltransferase Dnmt3a prefers to methylate the unmethylated DNA.
|Yokochi T,Robertson KD||The Journal of biological chemistry (277:11735)||2002|