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Simultaneous detection of expression of five genes in a whole-mount Drosophila embryo by fluorescence in situ hybridization (FISH) with five RNA probes. Red: sog labeled using aminoallyl UTP (Cat. No. A21663, A32765) and Alexa Fluor® 647 succinimidyl ester (Cat. No. A20006, A20106). Green: ind labeled with DNP, followed by rabbit anti–dinitrophenyl-KLH IgG antibody (Cat. No. A6430) prelabeled with the Zenon® Alexa Fluor® 555 Rabbit IgG Labeling Kit (Cat. No. Z25305). Blue: en labeled with biotin and detected with HRP–streptavidin and Alexa Fluor® 405 tyramide (TSA™ Kit ##39, T30952). Yellow: wg labeled with digoxigenin and detected with sheep anti–digoxigenin IgG antibody and Alexa Fluor® 594 Donkey Anti–Sheep IgG antibody (Cat. No. A11016). Magenta: msh labeled with fluorescein and detected with mouse anti–fluorescein/Oregon Green® IgG<sub>2a</sub> antibody (Cat. No. A6421) and Alexa Fluor® 488 Goat Anti–Mouse IgG antibody (Cat. No. A11001, A11029). Image contributed by Dave Kosman and Ethan Bier, University of California, San Diego.
|Tested species reactivity||Chem|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Storage buffer||PBS, pH 7.2|
|Contains||5mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Correlations between Photodegradation of Bisretinoid Constituents of Retina and Dicarbonyl Adduct Deposition.
A-6430 was used in immunohistochemistry to study retinal pigment epithelial lipofuscin as a source of the reactive species that covalently modify protein side chains.
|Zhou J,Ueda K,Zhao J,Sparrow JR||The Journal of biological chemistry (290:27215)||2015|
A major facilitator superfamily protein participates in the reddish brown pigmentation in Bombyx mori.
A-6430 was used in immunohistochemistry to report that mucK is required for normal body color pattern in insect larvae.
|Zhao Y,Zhang H,Li Z,Duan J,Jiang J,Wang Y,Zhan S,Akinkurolere RO,Xu A,Qian H,Miao X,Tan A,Huang Y||Journal of insect physiology (58:1397)||2012|
|Not Applicable||Not Cited||
Localization of cerebellin-2 in late embryonic chicken brain: implications for a role in synapse formation and for brain evolution.
A-6430 was used in immunohistochemistry to test the role of Cbln2 in synaptically connected neuronal populations.
|Reiner A,Yang M,Cagle MC,Honig MG||The Journal of comparative neurology (519:2225)||2011|
Intracellular oxidant activity, antioxidant enzyme defense system, and cell senescence in fibroblasts with trisomy 21.
A-6430 was used in western blot to determine if an imbalance exists in activities, mRNA, and protein expression of the antioxidant enzymes SOD1, SOD2, glutathione-peroxidase, and catalase in T21F cells
|Rodríguez-Sureda V,Vilches Á,Sánchez O,Audí L,Domínguez C||Oxidative medicine and cellular longevity (2015:null)||2015|
Impact of photocatalysis on fungal cells: depiction of cellular and molecular effects on Saccharomyces cerevisiae.
A-6430 was used in western blot to investigate the antimicrobial mechanisms during photocatalysis
|Thabet S,Simonet F,Lemaire M,Guillard C,Cotton P||Applied and environmental microbiology (80:7527)||2014|
TRF2 and lamin A/C interact to facilitate the functional organization of chromosome ends.
A-6430 was used in immunocytochemistry to investigate how the interaction between TRF2 and lamin A/C regulates chromosome structure
|Wood AM,Rendtlew Danielsen JM,Lucas CA,Rice EL,Scalzo D,Shimi T,Goldman RD,Smith ED,Le Beau MM,Kosak ST||Nature communications (5:null)||2014|
|Not Applicable||Not Cited||
Defective hematopoietic stem cell and lymphoid progenitor development in the Ts65Dn mouse model of Down syndrome: potential role of oxidative stress.
A-6430 was used in flow cytometry to assess the hematopoietic stem and lymphoid progenitor cell function in the Ts65Dn mouse model of Down syndrome.
|Lorenzo LP,Chen H,Shatynski KE,Clark S,Yuan R,Harrison DE,Yarowsky PJ,Williams MS||Antioxidants & redox signaling (15:2083)||2011|