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A mixed population of trinitrophenyl-sensitized and nonsensitized bovine pulmonary artery endothelial cells that have been probed with anti–dinitrophenyl-KLH antibody (Cat. no. A6430), incubated with rabbit complement and then stained using the LIVE/DEAD® Reduced Biohazard Viability/Cytotoxicity Kit ##1 (Cat. no. L7013). With this kit, the membrane-permeant SYTO® 10 dye labels the nucleic acids of live cells with green fluorescence, and the membrane-impermeant DEAD Red™ dye labels nucleic acids of membrane-compromised cells with red fluorescence. Subsequent aldehyde-based fixation inactivates pathogens without distorting the original staining pattern. This multiple-exposure image was acquired with bandpass optical filters appropriate for fluorescein and Texas Red® dyes.
|Tested species reactivity||Chem|
|Published species reactivity||Human , Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Storage buffer||PBS, pH 7.2|
|Contains||5mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Correlations between Photodegradation of Bisretinoid Constituents of Retina and Dicarbonyl Adduct Deposition.
A-6430 was used in immunohistochemistry to study retinal pigment epithelial lipofuscin as a source of the reactive species that covalently modify protein side chains.
|Zhou J,Ueda K,Zhao J,Sparrow JR||The Journal of biological chemistry (290:27215)||2015|
A major facilitator superfamily protein participates in the reddish brown pigmentation in Bombyx mori.
A-6430 was used in immunohistochemistry to report that mucK is required for normal body color pattern in insect larvae.
|Zhao Y,Zhang H,Li Z,Duan J,Jiang J,Wang Y,Zhan S,Akinkurolere RO,Xu A,Qian H,Miao X,Tan A,Huang Y||Journal of insect physiology (58:1397)||2012|
|Not Applicable||Not Cited||
Localization of cerebellin-2 in late embryonic chicken brain: implications for a role in synapse formation and for brain evolution.
A-6430 was used in immunohistochemistry to test the role of Cbln2 in synaptically connected neuronal populations.
|Reiner A,Yang M,Cagle MC,Honig MG||The Journal of comparative neurology (519:2225)||2011|
Intracellular oxidant activity, antioxidant enzyme defense system, and cell senescence in fibroblasts with trisomy 21.
A-6430 was used in western blot to determine if an imbalance exists in activities, mRNA, and protein expression of the antioxidant enzymes SOD1, SOD2, glutathione-peroxidase, and catalase in T21F cells
|Rodríguez-Sureda V,Vilches Á,Sánchez O,Audí L,Domínguez C||Oxidative medicine and cellular longevity (2015:null)||2015|
Impact of photocatalysis on fungal cells: depiction of cellular and molecular effects on Saccharomyces cerevisiae.
A-6430 was used in western blot to investigate the antimicrobial mechanisms during photocatalysis
|Thabet S,Simonet F,Lemaire M,Guillard C,Cotton P||Applied and environmental microbiology (80:7527)||2014|
TRF2 and lamin A/C interact to facilitate the functional organization of chromosome ends.
A-6430 was used in immunocytochemistry to investigate how the interaction between TRF2 and lamin A/C regulates chromosome structure
|Wood AM,Rendtlew Danielsen JM,Lucas CA,Rice EL,Scalzo D,Shimi T,Goldman RD,Smith ED,Le Beau MM,Kosak ST||Nature communications (5:null)||2014|