OriGene

DOCK2 Monoclonal Antibody (UMAB142), UltraMAB™

View all (20) DOCK2 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

DOCK2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of Jurkat cells using anti-DOCK2 mouse monoclonal antibody (UM800039, green, 1:100). Actin filaments were labeled with Alexa Fluor 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

DOCK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human lung cancer using anti-DOCK2 clone UMAB142 mouse monoclonal antibody at 1:200 dilution of mg/mL using Polink2 Broad HRP DAB for detection. UM800039 requires HIER with with citrate pH6.0 at 110°C for 3 min using pressure chamber/cooker. IHC staining shows tumor cells are negative however infiltrating lymphocytes strongly positive.

DOCK2 Antibody in Western Blot (WB) — Western blot analysis of RPMI8226 cell lysate (35 µg) by using anti-DOCK2 monoclonal antibody.

Product Details

UM800039

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-
Immunocytochemistry (ICC/IF)	1:150	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	UMAB142
Immunogen	Human recombinant protein fragment corresponding to amino acids 1544-1830 of human DOCK2 produced in E.coli. View immunogen
Conjugate	Unconjugated
Form	liquid
Concentration	0.5-1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Target Information

The DOCK2 gene encodes a hematopoietic cell-specific CDM family protein that is indispensable for lymphocyte chemotaxis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: dedicator of cyto-kinesis 2; Dedicator of cytokinesis protein 2; FLJ46592

Gene Aliases: DOCK2; IMD40; KIAA0209

UniProt ID: (Human) Q92608

Entrez Gene ID: (Human) 1794

Molecular Function: guanyl-nucleotide exchange factor

Function(s)

membrane raft polarization establishment of T cell polarity immunological synapse formation myeloid dendritic cell activation involved in immune response chemotaxis small GTPase mediated signal transduction actin cytoskeleton organization positive regulation of GTPase activity macropinocytosis positive thymic T cell selection negative thymic T cell selection alpha-beta T cell proliferation regulation of defense response to virus by virus positive regulation of phagocytosis

Process(es)

GTPase activator activity protein binding Rac guanyl-nucleotide exchange factor activity T cell receptor binding

DOCK2 Monoclonal Antibody (UMAB142), UltraMAB™

Product Details

UM800039

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Target Information

References (0)

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!