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|Tested species reactivity||Yeast|
|Published species reactivity||Yeast|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Yeast Dolichol phosphate mannose synthase|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 6 publications below|
Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A DNA-dependent protease involved in DNA-protein crosslink repair.
A-6429 was used in western blot to show that Wss1 is crucial for cell survival upon exposure to formaldehyde and topoisomerase 1-dependent DNA damage
|Stingele J,Schwarz MS,Bloemeke N,Wolf PG,Jentsch S||Cell (158:327)||2014|
Secretory vesicles deliver Cdc42p to sites of polarized growth in S. cerevisiae.
A-6429 was used in western blot to show that Cdc42p associates with secretory vesicles in vivo using S. cerevisiae
|Dighe SA,Kozminski KG||PloS one (9:null)||2014|
Proteomic profiling of autophagosome cargo in Saccharomyces cerevisiae.
A-6429 was used in western blot to describe a method for monitoring intact autophagosomes ex vivo.
|Suzuki K,Nakamura S,Morimoto M,Fujii K,Noda NN,Inagaki F,Ohsumi Y||PloS one (9:null)||2014|
Host acyl coenzyme A binding protein regulates replication complex assembly and activity of a positive-strand RNA virus.
A-6429 was used in western blot to investigate how vesicular spherules and stacks of appressed double-membrane layers are formed.
|Zhang J,Diaz A,Mao L,Ahlquist P,Wang X||Journal of virology (86:5110)||2012|
Accumulation of an antidepressant in vesiculogenic membranes of yeast cells triggers autophagy.
A-6429 was used in western blot to investigate the effects of sertraline/Zoloft® on the cell physiology of Saccharomyces cerevisiae.
|Chen J,Korostyshevsky D,Lee S,Perlstein EO||PloS one (7:null)||2012|
Intersection of the multivesicular body pathway and lipid homeostasis in RNA replication by a positive-strand RNA virus.
A-6429 was used in western blot to investigate the relationship between lipid synthesis and brome mosaic virus RNA replication.
|Wang X,Diaz A,Hao L,Gancarz B,den Boon JA,Ahlquist P||Journal of virology (85:5494)||2011|
CDGIE; dolichol monophosphate mannose synthase; Dolichol-phosphate mannose synthase; Dolichol-phosphate mannosyltransferase; Dolichyl-phosphate beta-D-mannosyltransferase; DPM synthase; Mannose-P-dolichol synthase; MPD synthase; MPDS
DPM1; P9705.3; SED3; YPR183W