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Published figure using DPM1 monoclonal antibody (Product # A-6429)
Figure 3 Co-purification of Cdc42p with low density, Sec4p-marked vesicles. A) Immunoblots of 100,000x g pellets of fractions collected from an 18-34% Nycodenz-sorbitol buoyant density gradient loaded with extracts prepared from equivalent OD 600 units of sec6-4 ts cells grown at 25degC or 37degC for 90 min. Each lane was loaded equivalent fraction volumes and, for the same probe, incubated with antibody of equivalent titer for the same amount of time. All blots are from the same fractionation, with the Sec4p and Bgl2p panels reprinted with permission from Alfaro et al .  (c) 2011 Traffic . All blots at an indicated temperature were prepared from the same fractions from the same gradient. B) Graph showing invertase activity across ""37degC"" buoyant density gradient shown in (A) and sucrose density across an unloaded gradient processed in parallel. Fraction densities were calculated based on a standard curve of Nycodenz-sorbitol concentration versus refractive index. C) Thin section electron micrographs showing vesicle association with a M500 Dynabead coated with mouse monoclonal anti-Sec4p antibody (left), but not with the same type of bead coated with mouse IgG (right), after incubation with a pooled, Sec4p-enriched, buoyant-density gradient fraction from sec6-4 ts cells grown at 37degC for 90 min (comparable to fractions 11-14 in (A)). D) Immunoblots of a pooled, Sec4p-enriched, low-density gradient fraction (S), after incubation with Protein G-Dynabeads (P) coated wit
FIGURE 7: Bck2 and Sdp1 associate with mitochondria during heat shock. (A) Some heat shock-induced Bck2 puncta colocalize with mitochondria. A plasmid expressing an RFP-tagged mitochondrial marker (Mito-RFP; pHCRED) was introduced into Bck2-GFP cells. All images represent single optical sections captured by spinning disk confocal microscopy 15 min after shifting cells to 39degC (heat shock). (B) The cell is presented as a three-dimensional (3D) model via Volocity software (PerkinElmer). Left, a merge/projection of 21 x 0.2 mum Z-sections. Middle and right, different angles of a 3D model of the same cell. See Supplemental Movie S2 for this model in rotation. (C) Sdp1 localizes to mitochondria in heat-shocked cells. Cells expressing Sdp1-GFP and Mito-RFP (FLY3570, plasmids pRS425-SDP1-GFP, pHCRED) were monitored after 15 min of heat stress, as described in A. (D) Immunoblots of organelle fractions of unstressed (22degC) and heat-shocked (39degC) Sdp1-GFP cells (see Materials and Methods ). Western blots are probed with antibodies to GFP, Tim23 (a mitochondrial marker), and Dpm1 (ER/microsome marker). Mitochondria are enriched in P2, microsomes and ER are enriched in P3, and S3 is enriched for cytosolic proteins. Note that Sdp1 is enriched in fraction P2 (mitochondria) of heat-shocked cells and not in P2 of unstressed cells. Some Sdp1 is also present in fraction P3 (ER/microsome), regardless of heat shock.
Western Blot (WB)
Host / Isotype
Yeast Dolichol phosphate mannose synthase
HEPES buffered saline
0.02% sodium azide
Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Protein Aliases: CDGIE; dolichol monophosphate mannose synthase; Dolichol-phosphate mannose synthase; Dolichol-phosphate mannosyltransferase; Dolichyl-phosphate beta-D-mannosyltransferase; DPM synthase; Mannose-P-dolichol synthase; MPD synthase; MPDS
Gene Aliases: DPM1; P9705.3; SED3; YPR183W
UniProt ID: (Baker's yeast) P14020
Entrez Gene ID: (Baker's yeast) 856313
Suggested Secondary Antibodies
Material safety data sheets (MSDS)