Immunofluorescence analysis of TRAIL-R2 / DR5 was done on 70% confluent log phase HeLa cell. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TRAIL-R2 / Rabbit Polyclonal Antibody (PA1957) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: C V(255) P E Q E M E V Q E P A E P T G(270)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2-3 ug/ml|
|Immunofluorescence (IF)||2-3 ug/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
PA1-957 detects death receptor 5 (DR5) from human and rat tissues and cells.
PA1-957 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~58 kDa protein representing DR5 from HEK293 cell lysate.
PA1-957 immunizing peptide corresponds to amino acid residues 255-270 of rat DR5. PA1-957 immunizing peptide (Cat. # PEP-048) is available for use in neutralization and control experiments.
DR5 is a recently identified death domain containing receptor for TRAIL, which mediates TRAIL induced apoptosis. DR5 is also known as Apo2, TRAIL-R2, TRICK2 and KILLER.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
BCL2L10 protein regulates apoptosis/proliferation through differential pathways in gastric cancer cells.
PA1-957 was used in western blot to investigate the role of BCL2L10 in cell cycle regulation in tumors
|Xu JD,Cao XX,Long ZW,Liu XP,Furuya T,Xu JW,Liu XL,De Xu Z,Sasaki K,Li QQ||The Journal of pathology (223:400)||2011|
Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors.
PA1-957 was used in western blot to investigate the regulation of apoptosis induced by TRAIL
|Sheridan JP,Marsters SA,Pitti RM,Gurney A,Skubatch M,Baldwin D,Ramakrishnan L,Gray CL,Baker K,Wood WI,Goddard AD,Godowski P,Ashkenazi A||Science (New York, N.Y.) (277:818)||1997|