Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
DVL2 Antibody (CF806832) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DVL2 (RC202233, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-DVL2. Positive lysates LY401405 (100 µg) and LC401405 (20 µg) can be purchased separately from OriGene.
Product Details
CF806832
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1B12
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-320 of human DVL2 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Disheveled DVL2 is a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: dishevelled 2 (homologous to Drosophila dsh); dishevelled, dsh homolog 2; Dishevelled-2; DSH homolog 2; Segment polarity protein dishevelled homolog DVL-2
Gene Aliases: DVL2
UniProt ID: (Human) O14641
Entrez Gene ID: (Human) 1856
neural tube closure
positive regulation of protein phosphorylation
outflow tract morphogenesis
transcription from RNA polymerase II promoter
segment specification
heart development
Wnt signaling pathway
convergent extension involved in neural plate elongation
cellular protein localization
hippo signaling
non-canonical Wnt signaling pathway
positive regulation of JUN kinase activity
positive regulation of GTPase activity
canonical Wnt signaling pathway involved in regulation of cell proliferation
positive regulation of transcription, DNA-templated
positive regulation of sequence-specific DNA binding transcription factor activity
protein oligomerization
canonical Wnt signaling pathway
Wnt signaling pathway, planar cell polarity pathway
positive regulation of protein tyrosine kinase activity
negative regulation of canonical Wnt signaling pathway
cochlea morphogenesis
planar cell polarity pathway involved in neural tube closure
positive regulation of canonical Wnt signaling pathway
beta-catenin destruction complex disassembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support