|Tested species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Recognizes Dab1 of 48 kDa, corresponding to amino acids 399-555 of mouse Dab1 GST fusion protein.|
|Contains||0.08% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||5 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-21178 detects Dab1 in Mouse samples.
PA1-21178 has been successfully used in Immunoprecipitation and Western Blot procedures. Suggested positive control for Western blot is 293 cells transfected with Dab1 HA tag and for immunoprecipitation is Dab1 knockout brain p14 lysated and Dab1 wild type brain p14 lysate. PA1-21178 detects a band of approximately 80 kDa.
PA1-21178 immunogen corresponds to Recognizes Dab1 of 48 kDa, corresponding to amino acids 399-555 of mouse Dab1 GST fusion protein..
Disruption of Dab1 results in aberrant migration of neurons during development and disorganization of laminar structures throughout the brain. Dab1 is thought to function as an adaptor molecule in signal transduction processes. It contains a protein-protein interaction (PI) similar to the phosphotyrosine binding domain of the Shc oncoprotein, it is phosphorylated by the Src protein tyrosine kinase, and it binds to SH2 binding domains in a phosphotyrosine dependent manner. DAB1 acts downstream of reelin in a signalling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.