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|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Dectin-2 (extracellular domain) Fc Fusion protein.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10 - 1:100|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Blocking Assay (BLOCK)||See 2 publications below|
This antibody does not stain brain microglia or meningeal macrophages.
This product is a low-endotoxin, preservative-free formulation.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
Dectin-2 is a C-type lectin expressed by dendritic cells, macrophages and inflammatory monocytes. It is a type II membrane receptor, with a short cytoplasmic tail, and a C-terminal C-type lectin domain. Dectin-2 is a receptor with specificity for high mannose structures and mediates cytokine production from DC and other antigen presenting cells. Though, Dectin-2 has no obvious signaling motifs attached to CP tail, reports indicate that Dectin-2 employs FcRg for internalization and to activate NF-kappaB pathway for cytokine production. Though it shares only 20-25% of homology with Dectin-1, it functions similarly as pattern recognition receptor (PRR) for fungal infections, to activate both innate (Syk-CARD9 innate signaling pathway) and adaptive immune responses (by activation of DC) to fungal infections. Thus Dectin-2 is a connector molecule of both innate and adaptive immunity functioning at DC level.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Dectin-2 regulates the effector phase of house dust mite-elicited pulmonary inflammation independently from its role in sensitization.
MA1-82675 was used in blocking or activating experiment to study the role of dectin-2 in modulating pulmonary inflammation following house dust mite challenge
|Parsons MW,Li L,Wallace AM,Lee MJ,Katz HR,Fernandez JM,Saijo S,Iwakura Y,Austen KF,Kanaoka Y,Barrett NA||Journal of immunology (Baltimore, Md. : 1950) (192:1361)||2014|
Dectin-2 mediates Th2 immunity through the generation of cysteinyl leukotrienes.
MA1-82675 was used in blocking or activating experiment to study the role of the glycan receptor Deptin-2 in immunity
|Barrett NA,Rahman OM,Fernandez JM,Parsons MW,Xing W,Austen KF,Kanaoka Y||The Journal of experimental medicine (208:593)||2011|
C-type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 10; C-type lectin domain family 6 member A; C-type lectin superfamily member 10; Clec6a; Clecsf10; DC-associated C-type lectin 2; dectin-2; dendritic cell-associated C-type lectin 2; natural killer complex lectin; Nkcl
Clec4n; Clec6a; Clecsf10; Dectin2; Nkcl