Immunohistochemistry was performed on 4% paraformaldehyde-fixed frozen mouse retina tissue sections. Tissues were blocked in 3% normal goat serum + 0.3% Triton X-100 for 1 hour at room temperature and probed with a Degenerative Spermatocyte Homolog 1 (DSH1) polyclonal antibody (Product # PA1-12912) at a dilution of 1:500 overnight at 4°C . Tissues were washed extensively with PBS. Detection was performed using a Cy3-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:500. Nuclei (blue) were stained with DAPI, and staining was visualized by fluorescence microscopy. Data courtesy of the Innovators Program.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the human DEGS1 protein.|
|Purification||Ammonium sulfate precipitation|
|Contains||0.08% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:200-1:500|
|Western Blot (WB)||1-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is placenta lysate.
The product of the DEGS1 gene is a member of the membrane fatty acid (sphingolipid delta 4 ) desaturase family. Reported to function by inserting double bonds at delta 4 position in fatty acids. DEGS1 is an intracellular enzyme. Overexpression of this gene inhibited biosynthesis of the EGF receptor, suggesting a possible role of a fatty acid desaturase in regulation biosynthetic processing of the EGF receptor. Two splice variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.