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Immunofluorescent analysis of Dengue Virus Type 1-4 showing staining in the cytoplasm of BHK-21 cells. BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH and stained using a Dengue Virus Type 1-4 monoclonal antibody (Product # MA1-27093) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Virus|
|Published species reactivity||Primate, Virus, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Mixture of dengue 1, 2, 3 and 4.|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Dot blot (DB)||Assay Dependent|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-27093 detects Dengue Virus 1+2+3+4 from all dengue serotype samples.
MA1-27093 has been successfully used in ELISA, dot blot and Western blot procedures.
The MA1-27093 immunogen is a mixture of dengue 1, 2, 3 and 4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Induced autophagy reduces virus output in dengue infected monocytic cells.
MA1-27093 was used in western blot to study the the reduction in virus output by U937 monocytes infected with dengue virus by the induction of autophagy
|Panyasrivanit M,Greenwood MP,Murphy D,Isidoro C,Auewarakul P,Smith DR||Virology (418:74)||2011|
Mutations of an antibody binding energy hot spot on domain III of the dengue 2 envelope glycoprotein exploited for neutralization escape.
MA1-27093 was used in ELISA to characterize the antibody binding epitopes on domain III of the dengue 2 envelope glycoprotein
|Gromowski GD,Roehrig JT,Diamond MS,Lee JC,Pitcher TJ,Barrett AD||Virology (407:237)||2010|
Characterization of dengue complex-reactive epitopes on dengue 3 virus envelope protein domain III.
MA1-27093 was used in ELISA to characterize the reactive epitopes for several monoclonal dengue virus antibodies
|Matsui K,Gromowski GD,Li L,Schuh AJ,Lee JC,Barrett AD||Virology (384:16)||2009|
Characterization of dengue virus complex-specific neutralizing epitopes on envelope protein domain III of dengue 2 virus.
MA1-27093 was used in ELISA to map the epitopes of five mature dengue virus-specific monoclonal antibodies
|Gromowski GD,Barrett ND,Barrett AD||Journal of virology (82:8828)||2008|